Growth Characterization of Bacillus sp. strain ZEID-14 on Acrylamide as the Sole Nitrogen Source
DOI:
https://doi.org/10.54987/jebat.v5i2.737Keywords:
Acrylamide, Bacillus sp., Bioremediation, Biodegradation, CharacterizationAbstract
Acrylamide is a major pollution in soil from the breakdown of pesticides additive. Slowly but steadily, the use of microbe-mediated acrylamide breakdown as a bioremediation approach has gained attention all around the world. At room temperature, the effect of the initial pH on bacterial growth shows that the optimum pH range was discovered to be between 6.5 and 7.5. The optimal growing temperature at pH 7.5 ranged from 25 to 35 oC. In a series of experiments using a 1.0 percent (w/v) starting concentration of various organic carbon sources, it was determined that fructose, glucose and sucrose all supported the greatest amount of cellular growth on acrylamide. Acrylamide concentration of 500 mg/L promoted the most rapid expansion of growth, while levels of 1500 mg/L and higher entirely stopped growth. Mercury at 2 ppm caused 83% of inhibition whilst other metal ions such as copper, cadmium, lead and chromium showed minimal inhibition of less than 20% . The concentration of acrylamide and the time it took for this bacterium to start growing show an inverse relationship. A lag time of 1-3 days was found as the content of acrylamide was raised from 100 to 1000 mg/L while growth was abolished at 1500 mg/L. The maximal growth rate increased as acrylamide concentrations increased, indicating an overall trend of increased toxicity.
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References
Mottram, DS, Wedzicha BL, Dobson AT. Acrylamide is formed in the Maillard reaction. Nature. 2002;419:448-9.
Zamora R, Delgado RM, Hidalgo FJ. Strecker aldehydes and ?-keto acids, produced by carbonyl-amine reactions, contribute to the formation of acrylamide. Food Chem. 2011;128(2):465-70.
Shukor MY, Gusmanizar N, Azmi NA, Hamid M, Ramli J, Shamaan NA, et al. Isolation and characterization of an acrylamide-degrading Bacillus cereus. J Enviromental Biol. 2009;30(1):57-64.
Sega GA, Valdivia Alcota RP, Tancongco CP, Brimer PA. Acrylamide binding to the DNA and protamine of spermiogenic stages in the mouse and its relationship to genetic damage. Mutat Res Mutagen Relat Subj. 1989 Aug 1;216(4):221-30.
Tyl RW, Friedman MA. Effects of acrylamide on rodent reproductive performance. Reprod Toxicol. 2003 Jan 1;17(1):1-13.
Backer LC, Dearfield KL, Erexson GL, Campbell JA, Westbrook?Collins B, Allen JW. The effects of acrylamide on mouse germ-line and somatic cell chromosomes. Environ Mol Mutagen. 1989;13(3):218-26.
Spencer P, Schaumburg HH. Nervous system degeneration produced by acrylamide monomer. Environ Health Perspect. 1975 Jun 1;11:129-33.
Eikmann T, Herr C. How dangerous is actually acrylamide exposure for the population. Umweltmed Forsch Prax. 2002;7(6):307-8.
Pruser KN, Flynn NE. Acrylamide in health and disease. Front Biosci - Sch. 2011;3 S(1):41-51.
Pennisi M, Malaguarnera G, Puglisi V, Vinciguerra L, Vacante M, Malaguarnera M. Neurotoxicity of acrylamide in exposed workers. Int J Environ Res Public Health. 2013;10(9):3843-54.
Hagmar L, Törnqvist M, Nordander C, Rosén I, Bruze M, Kautiainen A, et al. Health effects of occupational exposure to acrylamide using hemoglobin adducts as biomarkers of internal dose. Scand J Work Environ Health. 2001;27(4):219-26.
Igisu H, Goto I, Kawamura Y, Kato M, Izumi K. Acrylamide encephaloneuropathy due to well water pollution. J Neurol Neurosurg Psychiatry. 1975;38(6):581-4.
Wampler DA, Ensign SA. Photoheterotrophic metabolism of acrylamide by a newly isolated strain of Rhodopseudomonas palustris. Appl Environ Microbiol. 2005;71(10):5850-7.
Buranasilp K, Charoenpanich J. Biodegradation of acrylamide by Enterobacter aerogenes isolated from wastewater in Thailand. J Environ Sci. 2011;23(3):396-403.
Charoenpanich J, Tani A. Proteome analysis of acrylamide-induced proteins in a novel acrylamide-degrader Enterobacter aerogenes by 2D electrophoresis and MALDI-TOF-MS. Chiang Mai Univ J Nat Sci. 2014;13(1):11-22.
Gusmanizar N, Shukor Y, Ramli J, Syed MA. Isolation and characterization of an acrylamide-degrading Burkholderia sp. strain DR.Y27. J Ris Kim. 2015 Feb 11;2(1):34.
Yu F, Fu R, Xie Y, Chen W. Isolation and characterization of polyacrylamide-degrading bacteria from dewatered sludge. Int J Environ Res Public Health. 2015;12(4):4214-30.
Bedade DK, Singhal RS. Biodegradation of acrylamide by a novel isolate, Cupriavidus oxalaticus ICTDB921: Identification and characterization of the acrylamidase produced. Bioresour Technol. 2018 Aug 1;261:122-32.
Aisami A, Gusmanizar N. Characterization of an acrylamide-degrading bacterium isolated from hydrocarbon sludge. Bioremediation Sci Technol Res. 2019 Dec 28;7(2):15-9.
Othman AR, Rahim MBHA. Modelling the Growth Inhibition Kinetics of Rhodotorula sp. strain MBH23 (KCTC 11960BP) on Acrylamide. Bioremediation Sci Technol Res. 2019 Dec 28;7(2):20-5.
Rusnam, Gusmanizar N. An Acrylamide-degrading Bacterial Consortium Isolated from Volcanic Soil. J Biochem Microbiol Biotechnol. 2021 Dec 31;9(2):19-24.
Rusnam, Gusmanizar N. Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil. J Environ Bioremediation Toxicol. 2022 Aug 5;5(1):32-7.
Adnan M, Abu Zeid I, Ahmad SA, Effendi Halmi M, Abdullah S, Shukor M. A Molybdenum-reducing Bacillus sp. Strain Zeid 14 in Soils from Sudan that Could Grow on Amides and Acetonitrile. Malays J Soil Sci. 2016 Jan 1;20:111-34.
Jonston JJ, Borden RC, Barlaz MA. Anaerobic biodegradation of alkylbenzenes and trichloroethylene in aquifer sediment down gradient of a sanitary landfill. J Contam Hydrol. 1996;23(4):263-83.
Baek SH, Kim KH, Yin CR, Jeon CO, Im WT, Kim KK, et al. Isolation and characterization of bacteria capable of degrading phenol and reducing nitrate under low-oxygen conditions. Curr Microbiol. 2003;47(6):462-6.
Egorova K, Trauthwein H, Verseck S. Purification and properties of an enantioselective and thermoactive amidase from the thermophilic actinomycete Pseudonocardia thermophila. Appl Microbiol Biotechnol. 2004;38-45.
Rahim MBH, Syed MA, Shukor MY. Isolation and characterization of an acrylamide-degrading yeast Rhodotorula sp . strain MBH23 KCTC 11960BP. J Basic Microbiol. 2012;52(5):573-81.
Rogacheva SM, Ignatov OV. The Respiratory Activity of Rhodococcus rhodochrous M8 Cells Producing Nitrile-Hydrolyzing Enzymes. Appl Biochem Microbiol. 2001;37(3):282-6.
Shukor MY, Ahmad SA, Nadzir MMM, Abdullah MP, Shamaan NA, Syed MA. Molybdate reduction by Pseudomonas sp . strain DRY2. J Appl Microbiol. 2010;108:2050-8.
Shen S min, Wan T jou, Hwang H yuan. Biocatalysis and Agricultural Biotechnology Enhancement of degradation of acrylamide coupled with salad oil by Pseudomonas aeruginosa DS-4 using incubation periods. Biocatal Agric Biotechnol. 2012;1(2):110-4.
Wakaizumi M, Yamamoto H, Fujimoto N, Ozeki K. Acrylamide degradation by filamentous fungi used in food and beverage industries. J Biosci Bioeng. 2009;108(5):391-3.
Cha M, Chambliss GH. Characterization of Acrylamidase Isolated from a Newly Isolated Acrylamide-Utilizing Bacterium , Ralstonia eutropha AUM-01. Curr Microbiol. 2011;671-8.
Shukor MY, Gusmanizar N, Ramli J, Shamaan NA, Maccormack WP, Syed MA. Isolation and characterization of an acrylamide-degrading Antarctic bacterium. J Enviromental Biol. 2009;30(1):107-12.
Amor L, Kennes C, Veiga MC. Kinetics of inhibition in the biodegradation of monoaromatic hydrocarbons in presence of heavy metals. Bioresour Technol. 2001 Jun 1;78(2):181-5.
Gopinath KP, Kathiravan MN, Srinivasan R, Sankaranarayanan S. Evaluation and elimination of inhibitory effects of salts and heavy metal ions on biodegradation of Congo red by Pseudomonas sp. mutant. Bioresour Technol. 2011;102(4):3687-93.
Roane TM, Josephson KL, Pepper IL. Dual-Bioaugmentation Strategy To Enhance Remediation of Cocontaminated Soil. Appl Environ Microbiol. 2001 Jul;67(7):3208-15.
Hettiarachchi GM, Pierzynski GM, Ransom MD. In situ stabilization of soil lead using phosphorus and manganese oxide. Environ Sci Technol. 2000;34(21):4614-9.
Babich H, Stotzky G. Effect of Cadmium on Fungi and on Interactions Between Fungi and Bacteria in Soil: Influence of Clay Minerals and pH. Appl Environ Microbiol. 1977 May;33(5):1059-66.
Kamel Z. Toxicity of cadmium to twoStreptomyces species as affected by clay minerals. Plant Soil. 1986 Jun 1;93(2):195-203.
Kulkarni NH, Muley AB, Bedade DK, Singhal RS. Cross-linked enzyme aggregates of arylamidase from Cupriavidus oxalaticus ICTDB921: process optimization, characterization, and application for mitigation of acrylamide in industrial wastewater. Bioprocess Biosyst Eng [Internet]. 2019; Available from: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075085765&doi=10.1007%2fs00449-019-02240-4&partnerID=40&md5=12e064000a11176469878181f8642894
Bedade DK, Muley AB, Singhal RS. Magnetic cross-linked enzyme aggregates of acrylamidase from Cupriavidus oxalaticus ICTDB921 for biodegradation of acrylamide from industrial waste water. Bioresour Technol. 2019;272:137-45.
Bedade DK, Singhal RS. Isolation and Characterization of Acrylamidase from Arthrobacter sp. DBV1 and Its Ability to Biodegrade Acrylamide. Appl Biochem Biotechnol. 2017;182(2):570-85.
Lakshmikandan M, Sivaraman K, Elaiya Raja S, Vasanthakumar P, Rajesh RP, Sowparthani K, et al. Biodegradation of acrylamide by acrylamidase from Stenotrophomonas acidaminiphila MSU12 and analysis of degradation products by MALDI-TOF and HPLC. Int Biodeterior Biodegrad. 2014;94:214-21.
Emmanuel Joshua Jebasingh S, Lakshmikandan M, Rajesh RP, Raja P. Biodegradation of acrylamide and purification of acrylamidase from newly isolated bacterium Moraxella osloensis MSU11. Int Biodeterior Biodegrad. 2013;85:120-5.
Syed MA, Ahmad SA, Kusnin N, Shukor MYA. Purification and characterization of amidase from acrylamide-degrading bacterium Burkholderia sp. strain DR.Y27. Afr J Biotechnol. 2012;11(2):329-36.
Cha M, Chambliss GH. Characterization of acrylamidase isolated from a newly isolated acrylamide-utilizing bacterium, Ralstonia eutropha AUM-01. Curr Microbiol. 2011;62(2):671-8.
Rahman MFA, Yasid NA, Ahmad SA, Shamaan NA, Shukor MY. Characterization of molybdenum-reduction by an acrylamide-degrading Antarctic bacterium. In 10-3 Midori-cho, Tachikawa, Tokyo, Japan: National Institute of Polar Research (NIPR); 2018. Available from: http://id.nii.ac.jp/1291/00015258/
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