Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil
DOI:
https://doi.org/10.54987/jebat.v5i1.678Keywords:
Acrylamide, Pseudomonas, Volcanic soils, Acrylamide-degrading, sumatraAbstract
Due to the fact that it breaks down into acrylamide over time, polyacrylamide is one of the most important sources of acrylamide in soil. As a strategy for bioremediation, the breakdown of acrylamide by the action of microbes has seen a gradual but consistent increase in attention all over the world. In this work, a bacterium, tentatively identified as Pseudomonas sp. strain Neni-12 that had been isolated from volcanic soil showed the ability to grow on acrylamide. The acrylamide-degrading bacterium grew best in the presence of glucose with acrylamide as the sole nitrogen source. At concentrations of acrylamide ranging from 400 to 600 mg/L, the organisms saw the greatest amount of growth, where ANOVA analysis shows no difference among these temperatures; however, growth was entirely halted at concentrations of 800 mg/L and above. The optimum pH was at 7.0, and growth was maximum between 25 and 35 °C. The bacterium is also capable of growing while using acetamide as the only source of nitrogen. An acrylamide-degrading bacterium that was isolated from volcanic soil is reported for the very first time here.
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