Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State

Authors

  • Hussaini Shettima Department of Microbiology, Faculty of Science, University of Maiduguri, P.M.B 1069 Borno State, Nigeria.
  • Ibrahim Alkali Allamin Department of Microbiology, Faculty of Science, University of Maiduguri, P.M.B 1069 Borno State, Nigeria.
  • Nasir Halima Department of Pure and Applied Chemistry Faculty of Science, University of Maiduguri, P.M.B 1069 Borno State, Nigeria.
  • Haruna Yahya Ismail Department of Microbiology, Faculty of Science, University of Maiduguri, P.M.B 1069 Borno State, Nigeria.
  • Yusuf Musa Department of Microbiology, Faculty of Science, University of Maiduguri, P.M.B 1069 Borno State, Nigeria.

DOI:

https://doi.org/10.54987/jebat.v4i2.632

Keywords:

Hydrocarbon, Bacteria, Mechanical workshops, Maiduguri, Soil

Abstract

The study was conducted to determine the distribution of hydrocarbon utilizing bacteria in spent engine oil (SEO) contaminated soil. Five mechanical workshops within Maiduguri Metropolis. Five bulk soil sample comprising of one each of the five sites; the sites are Leventis Area on Kashim Ibrahim Way, Damboa Raod, Boiler (commonly called Bola) near Maiduguri Monday Market, Ngomari, on Kano Road,  and 1000 Housing Estate, also on Kano Road. Nutrient agar was used, or isolation and enumeration total heterotrophic bacteria and Mineral salt agar was used for the isolation and enumeration of hydrocarbon utilizing bacteria. The result shows THB (92.0x104) at Damboa road (DR) to as higher and (78.8x104) at Bola area (BL) while HUB (2.0x104) at Damboa road (DR) and as high as (9.3x104) at Bola area (BL). The bacterial species isolated were species of Bacillus licheniformis, Bacillus subtilis, Bacillus coagulans, Bacillus alvei, Bacillus cereus, Bacillus lentus other are Pseudomonas aeruginosa, Klebsiella pneumonia. Bacillus licheniformis, and Bacillus subtilis are the most occurring bacterial isolates identified. The ability of those bacterial isolate to degrade hydrocarbon buoyantly will help in remediation of oil polluted environments.

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Published

2021-12-31

How to Cite

Shettima, H., Allamin, I. A., Halima, N., Ismail, H. Y., & Musa, Y. (2021). Isolation and Characterization of Hydrocarbon-degrading Bacteria in Soils of Mechanical Workshops in Maiduguri, Borno State. Journal of Environmental Bioremediation and Toxicology, 4(2), 35–38. https://doi.org/10.54987/jebat.v4i2.632

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