The Development Of An Inhibitive Assay For Heavy Metals Using The Acetylcholinesterase From Periophtalmodon schlosseri

Authors

  • Ahmad Siti Aqlima University Putra Malaysia

DOI:

https://doi.org/10.54987/jebat.v1i1.29

Keywords:

Periophtalmodon schlosseri, Acetylcholinesterase, Copper, Mercury and cadmium

Abstract

Acetylcholinesterase (AChE) assay is among the most rapid, simplest and economic assay in existence. AChE is known to be inhibited strongly by heavy metals aside from insecticides. Its use as an inhibitive assay for heavy metals is less well known. We report on the AChE from Periophtalmodon schlosseri as a sensitive assay for heavy metal ions. Heavy metals exhibited exponential decay type inhibition curves with calculated IC50 for copper, mercury, chromium and arsenic at 0.088, 0.371, 0.112 and 0.141 mg l-1, respectively. The LOD for copper, mercury, chromium and arsenic were 0.093, 0.126, 0.099 and 0.127 mg l-1, respectively. The LOQ for copper, mercury, chromium and arsenic were 0.001, 0.209, 0.103 and 0.219 mg l-1, respectively. The IC50sfor these heavy metals are lower than the values for immobilised urease, Daphnia magna, MicrotoxTM,rainbow trout, papain and bromelain assays.

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Published

2014-01-02

How to Cite

Siti Aqlima, A. (2014). The Development Of An Inhibitive Assay For Heavy Metals Using The Acetylcholinesterase From Periophtalmodon schlosseri. Journal of Environmental Bioremediation and Toxicology, 1(1), 20–24. https://doi.org/10.54987/jebat.v1i1.29

Issue

Vol. 1 No. 1 (2013)

Section

Articles

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