Dialysis Tubing Experiment Showed that Molybdenum Reduction in S. marcescens strain DrY6 is Mediated by Enzymatic Action

Authors

  • Mohd Yunus Shukor University Putra Malaysia

DOI:

https://doi.org/10.54987/jebat.v1i1.28

Keywords:

Reduction, Molybdenum, Molybdenum blue, S. Marcescens, dialysis tubing

Abstract

Reduction of metal ions by microbes has long been afflicted by the contribution of abiotic and nonabiotic metal-reducing chemicals. The discrimination between enzymatic and chemical action upon metal must be solved to present a clear reduction mechanism. In this work, a previously developed method using dialysis tubing was employed to study the contribution of these agents upon molybdenum reduction to molybdenum blue in S. marcescens strain Dr.Y6. We discovered that approximately 95 % of molybdenum blue formed was found in the inside of the dialysis tubing. This suggests that the reduction of molybdate by this bacterium requires the presence of cells or mediated enzymatically.

References

Bautista, E.M and M. Alexander: Reduction of inorganic compounds by soil microorganisms. Soil Sci. Soc. Am. Proc., 36, 918–920 (1972).

Campbell, M.A., A.D. Campbell and D.B. Villaret: Molybdate reduction by Eschericia coli K-12 and its chl mutants. Proc. Nat. Acad. Sci. USA., 82, 227–231 (1985).

Capaldi, A. and B. Proskauer: Beitrage Zur Kenntnis der Siurebildung bei Typhusbacillen und Bacterium coli, Zeitschr. F. Hyg. u Infektionskrankh., 23, 452–474 (1896).

Davis, G.K: Molybdenum. In: Metals and their compounds in the environment, occurrence, analysis and biological relevance. (Ed.: E. Merian). VCH Weinheim, New York (1991).

Demir, G., H.K. Ozcan, N. Tufekci and M. Borat: Decolorization of Remazol Yellow RR Gran by white rot fungus Phanerochaete chrysosporium. J. Environ. Biol., 28(4), 813–817 (2007).

Ghani, B., M. Takai, N.Z. Hisham, N. Kishimito, M.I.A. Ismail, T. Tano and T. Sugio: Isolation and characterization of a Mo6+-reducing bacterium. Appl. Environ. Microbiol., 59, 1176–1180 (1993).

Ghosh, T.K: Global environmental problems. J. Environ. Biol., 29(2), (2008).

Hem, J.D: Chemical factors that influence the availability of iron and manganese in aqueous systems. Geol. Soc. Am. Bull., 83, 443–450 (1972).

Jan, A.: La reduction biologique du molybdate d'ammonium par les bactéries du genre Serratia. (The biological reduction of ammonium molybdate by the bacterium from the genus Serratia). Bull. Sci. Pharmacol., 46, 336–339 (1939).

Marchal, J.G. and T.H. Gerard: Etude du pouvoir reducteur de quelque souches de colibacille sur le molybdate d'ammoniaque (Study of the reduction capacity of E. coli on ammonia molybdate). Trav. Lab. Microbiol. Fac. Pharm. Nancy., 6, 11–23 (1948).

Munch, J.C. and J.C.G. Ottow: Reductive transformation mechanism of ferric oxides in hydromorphic coils. Environ. Biogeochem. Ecol. Bull. (Stockholm), 35, 383–394 (1983).

Pant, D., A. Singh, Y. Satyawali and R.K. Gupta: Effect of carbon and nitrogen source amendment on synthetic dyes decolourizing efficiency of white-rot fungus, Phanerochaete chrysosporium. J. Environ. Biol., 29(1), 79–84 (2008).

Rahman, M.F.A., M.Y. Shukor, Z. Suhaili, S. Mustafa, N.A. Shamaan and M.A. Syed: Reduction of Mo(VI) by the bacterium Serratia sp. strain DRY5. J. Environ. Biol., 30(1), (2009).

Sahu, R.K., S. Katiyar, J. Tiwari and G.C. Kisku: Assessment of drain water receiving effluent from tanneries and its impact on soil and plants with particular emphasis on bioaccumulation of heavy metals. J. Environ. Biol., 28(3), 685–690 (2007).

Shukor, M.Y., N.A. Shamaan, M.A. Syed, C.H. Lee and M.I.A Karim: Isolation and characterization of molybdenum blue from Enterobacter cloacae Strain 48. Asia Pac. J. Mol. Biol. Biotechnol., 8(2), 167–172 (2000).

Shukor, M.Y., M.A. Syed, C.H. Lee, M.I.A. Karim and N.A. Shamaan: A method to distinguish between chemical and enzymatic reduction of molybdenum in Enterobacter cloacae strain 48. Malaysian J. Biochem., 7, 71–72 (2002).

Shukor, M.Y., S.H.M. Habib, M.F.A Rahman, H. Jirangon, M.P.A. Abdullah, N.A. Shamaan and M.A. Syed: Hexavalent molybdenum reduction to mo-blue by S. marcescens strain Dr.Y6. Appl. Biochem. Biotechnol., 149(1), 33–43 (2008).

Shukor, M.Y., W.S.W. Husin, M.F.A. Rahman, N.A. Shamaan and M.A. Syed: Isolation and characterization of an SDS-degrading Klebsiella oxytoca. J. Environ. Biol., 30(1), (2009a).

Shukor, M.Y., N. Gusmanizar, J. Ramli, N.A. Shamaan W.P. MacCormack and M.A. Syed: Isolation and characterization of an acrylamide-degrading Antarctic Bacterium. J. Environ. Biol., 30(1), (2009b).

Shukor, M.Y., N. Gusmanizar, N.A. Azmi, M. Hamid, J. Ramli, N.A. Shamaan and M.A. Syed: Isolation and characterization of an acrylamide-degrading Bacillus cereus. J. Environ. Biol., 30(1), (2009c).

Shukor, M.Y., N.A.A. Hassan, A.Z. Jusoh, N. Perumal, N.A. Shamaan, W.P. MacCormack and M.A. Syed: Isolation and characterization of a Pseudomonas diesel-degrading strain from Antarctica. J. Environ. Biol., 30(1), (2009d).

Shukor, M.Y., F.A. Dahalan, A.Z. Jusoh, R. Muse, N.A. Shamaan and M.A. Syed: Characterization of a diesel-degrading strain isolated from a hydrocarbon-contaminated site. J. Environ. Biol., 30(1), (2009e).

Shukor, M.Y., N.A. Bakar, A.R. Othman, I. Yunus, N.A. Shamaan and M.A. Syed: Development of an inhibitive enzyme assay for copper. J. Environ. Biol., 30(1), (2009f).

Shukor, M.Y., N.A. Baharom, N.A. Masdor, M.P.A. Abdullah, N.A. Shamaan, J.A. Jamal and M.A. Syed: The development of an inhibitive determination method for zinc using a serine protease. J. Environ. Biol., 30(1), (2009g).

Sidgwick, N.V: The chemical elements and their compounds. Clarendon Press, Oxford. (1984).

Srivastava, R.K., K.K. Yadav and S.P. Trivedi: Devicyprin induced gonadal impairment in a freshwater food fish, Channa punctatus (Bloch). J. Environ. Biol., 29(2), 187–191 (2008).

Sugio, T., Y. Tsujita, T. Katagiri, K. Inagaki and T. Tano: Reduction of Mo6+ with elemental sulfur by Thiobacillus ferrooxidans. J. Bacteriol., 170(12), 5956–5959 (1988).

Syed, M.A., H.K. Sim, A. Khalid and M.Y. Shukor: A simple method to screen for azo-dye-degrading bacteria. J. Environ. Biol., 30(1), (2009).

Tham, L.G., N. Perumal, M.A. Syed, N.A. Shamaan and M.Y. Shukor: Assessment of Clarias batrachus as a source of acetylcholinesterase (AChE) for the detection of insecticides. J. Environ. Biol., 30(1), (2009).

Woolfolk, C.A. and H.R. Whiteley: Reduction of inorganic compounds with molecular hydrogen by Micrococcus lactilyticus. I. Stoichiometry with compounds of arsenic, selenium, tellurium, transition and other elements. J. Bacteriol., 84, 647–658 (1962).

Yong, N.K., M. Oshima, R.C. Blake and T. Sugio: Isolation and some properties of an iron-oxidizing bacterium Thiobacillus ferrooxidans resistant to molybdenum ion. Biosci. Biotechnol. Biochem., 61, 1523–1526 (1997).

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Published

2014-01-02

How to Cite

Shukor, M. Y. (2014). Dialysis Tubing Experiment Showed that Molybdenum Reduction in S. marcescens strain DrY6 is Mediated by Enzymatic Action. Journal of Environmental Bioremediation and Toxicology, 1(1), 25–27. https://doi.org/10.54987/jebat.v1i1.28

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