Antibacterial Activity of Aloe Vera Extracts on Some Clinical Bacterial Isolates

Authors

  • Musa Usman Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.
  • A.H., Jauro Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.
  • Adamu Maina Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.
  • M.M. Ahmad Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.
  • A.U. Sumayyah Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.

DOI:

https://doi.org/10.54987/jebat.v7i2.1053

Keywords:

Antibacterial activity, Aloe vera plant, Clinical isolates, zone of inhibition, Resistance strains

Abstract

Aloe is a plant that is used against skin irritation, skin exposure to UV and gamma radiation, scalds, sunburn wounds, eczema, psoriasis, acne, dermatitis, ulcers, and to stimulate cell regeneration. This study aimed to determine the antibacterial activity of ethanolic and gel extracts of Aloe vera against certain clinical bacterial isolates. The extracts were screened for phytochemicals, and their antibacterial activities were determined using the well diffusion method at concentrations of 50, 100, 200, and 400 mg/mL. The result of phytochemical screening indicates the presence of alkaloids, flavonoids, tannins, anthraquinones, and steroids in both extracts. The result of the antibacterial assay revealed that both ethanolic and gel extracts of Aloe vera were active against E. coli at different concentrations, with zones of inhibition ranging from 25.5 mm to 20 mm. However, S. aureus shows resistance to the different concentrations of the extracts. Findings show that S. aureus was sensitive to ciprofloxacin, while resistant to Augmentin. Thus, the observed antibacterial resistance confirms S. aureus as a multi-drug-resistant strain, thereby justifying its resistance to the Aloe vera plant. This study suggests that the Aloe vera plant contains antibacterial properties.

References

Ali M. Antimicrobial Activities of Aqueous and Methanolic Extracts from Salvia officinalis and Salix acmophylla Used in the treatment of wound infection isolates. Ibn Al-Haytham J Pure Appl Sci. 2017;23(3):25-39.

Arbab S, Ullah H, Weiwei W, Wei X, Ahmad SU, Wu L, et al. Comparative study of antimicrobial action of aloe vera and antibiotics against different bacterial isolates from skin infection. Vet Med Sci. 2021;7(5):2061-7.

Mohd Nizam NH, Mohammad Rawi NF, Mhd Ramle SF, Abd Aziz A, Abdullah CK, Rashedi A, et al. Physical, thermal, mechanical, antimicrobial and physicochemical properties of starch based film containing aloe vera: a review. J Mater Res Technol. 2021 Nov 1;15:1572-89.

Cheung GYC, Bae JS, Otto M. Pathogenicity and virulence of Staphylococcus aureus. Virulence. 2021 Dec 31;12(1):547-69.

Linz MS, Mattappallil A, Finkel D, Parker D. Clinical Impact of Staphylococcus aureus Skin and Soft Tissue Infections. Antibiotics. 2023 Mar;12(3):557.

Kaper JB, Nataro JP, Mobley HLT. Pathogenic Escherichia coli. Nat Rev Microbiol. 2004 Feb;2(2):123-40.

Hunde M, Tafese W, Abdeta T, Addis M, Pal M. Studies on the Evaluation of Antibacterial Activities of Aloe barbadensis and Ocimum gratissimum Against Selected Zoonotic Bacterial Strains. Vol. 10, Indian Journal of Veterinary Public Health. 2024. p. 53.

Tulini FL, Hymery N, Haertle T, Blay GL, Martinus DE. Screening for antimicrobial and proteolytic activities of lactic acid bacteria isolated from cow, buffalo and goat milk and cheeses marketed in the southeast region of Brazil. J Dairy Res. 2016;83(1):115-24.

Awuchi C. Medicinal Plants and Herbal Medicines in Africa. In 2023.

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey's Manual of Determinative Bacteriology. 9th ed. 428 East Preston Street, Baltimore, Maryland 21202, USA: Lippincott Williams & Wilkins; 1994.

Sakran khawla A, Raharjo D, Mertaniasih NM. Antimicrobial Activities of Laurus nobilis Leaves Ethanol Extract on Staphylococcus aureus, Salmonellae typhi, and Escherichia coli. Indones J Trop Infect Dis. 2021 Aug 31;9(2):119-24.

Matin M. A review study on the pharmacological effects and mechanism of action of tannins. Eur J Pharm Med Res. 2021 July 2;8:05-10.

Duwiejua M, Woode E, Obiri D. Pseudo-akuammigine, an alkaloid from Picralima nitida seeds, has anti-inflammatory and analgesic actions in rats. J Ethnopharmacol. 2002 June 1;81:73-9.

Gary YG, Hammuel C, Adeyemi M, Magomya A, Agbajp A, Shallangwa G. Phytochemical screening and a comparative study of antibacterial activity of Aloe vera green rind, gel and leaf pulp extracts. Int Res J Microbiol. 2011 Nov 1;2(10):382-6.

Christian Aniekwu C, Okafor U, Onyeneho V. The Antibacterial Effect of Aloe Vera Gel Extract against Escherichia coli, Salmonella typhi and Staphylococcus aureus Isolated from Gastrointestinal Tract of Poultry Birds. Int J Res Innov Appl Sci. 2024 Jan 12;8:152-8.

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Published

2024-12-25

How to Cite

Usman, M., Jauro, A., Maina, A., Ahmad, M., & Sumayyah, A. . (2024). Antibacterial Activity of Aloe Vera Extracts on Some Clinical Bacterial Isolates. Journal of Environmental Bioremediation and Toxicology, 7(2), 114–116. https://doi.org/10.54987/jebat.v7i2.1053

Issue

Vol. 7 No. 2 (2024)

Section

Articles

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