Characterization of diesel-degrading enzymes from Acinetobacter sp. strain DRY12

Authors

  • Mohd Yunus Shukor Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia
  • Farrah Aini Dahalan Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Shamala Salvamani Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Arif Zaidi Jusoh Food Technology Research Centre, Malaysian Agriculture and Research Development Institute, Po. Box 1230, 50774 Kuala Lumpur, Malaysia.
  • Nor Aripin Shamaan Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, 13th Floor, Menara B, Persiaran Mpaj, Jalan Pandan Utama, Pandan Indah, 55100 Kuala Lumpur, Malaysia.
  • Mohd Arif Syed Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v1i1.9

Keywords:

diesel-degrading, Acinetobacter sp., terminal oxidation, alcohol dehydrogenase, aldehyde dehydrogenase, diesel-degrading, Acinetobacter sp., Terminal Oxidation

Abstract

The purpose of this work was to elucidate the type of metabolic pathway involved in the degradation of diesel by a diesel-degrading bacterium. The isolate was tentatively identified as Acinetobacter sp. strain dry12 based on partial 16s rDNA molecular phylogeny and Biolog® GN microplate panels and Microlog® database. Crude extract of the bacterium showed the presence of alkane-oxidizing, alcohol dehydrogenase and aldehyde dehydrogenase activities. The presence of these enzymes was further confirmed by the detection of 1-decanol, 1-decanal and 1-decanoic acid in the respective enzyme reaction mixtures. GC-MS fingerprint of several fatty acids detected in the growth media also suggested a terminal attack on diesel.

Downloads

Published

2013-12-31

How to Cite

Shukor, M. Y., Dahalan, F. A., Salvamani, S., Jusoh, A. Z., Shamaan, N. A., & Syed, M. A. (2013). Characterization of diesel-degrading enzymes from Acinetobacter sp. strain DRY12. Bioremediation Science and Technology Research (e-ISSN 2289-5892), 1(1), 15–18. https://doi.org/10.54987/bstr.v1i1.9

Issue

Vol. 1 No. 1 (2013)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).