Isolation and Growth Characterization of an Acrylamide-degrading E. cloacae strain UPM2021a Isolated from a Paddy Field
DOI:
https://doi.org/10.54987/bstr.v10i2.739Keywords:
Acrylamide, E. cloacae, Bioremediation, Paddy field, CharacterizationAbstract
Acrylamide is a suspected carcinogen and a global pollutant. The presence of acrylamide in the soil is a major source of this chemical. Microbe-mediated acrylamide breakdown as a bioremediation technique is gaining popularity across the world. Several bacteria capable of digesting acrylamide have been identified in paddy field soils. The best isolate was a bacterium identified tentatively as E. cloacae strain UPM2021a based on cultural, colony morphology and biochemical tests. According to early studies, ideal growth parameters included a pH range of 6.5 and 7.5 and a temperature range of 25 to 35 degrees Celsius. Acrylamide dosages of up to 2500 mg/L were explored as a single nitrogen supply. The greatest growth occurs between 300 and 1000 mg/L of acrylamide, resulting in an approximate nett growth of 3 log CFU/mL when compared to the control. Growth was practically tolerated at 1700 mg/L, and growth stopped entirely at concentrations above 2000 mg/L. Toxic heavy metals such as mercury, copper, chromium, and cadmium hampered acrylamide development with mercury being the strongest inhibitor whilst other metal ions such as copper, cadmium, and chromium show from 30 to 50% inhibition whilst lead was the least inhibiting. The relatively high tolerant of acrylamide makes this bacterium suitable for remediation of soil contaminated with acrylamide whilst its sensitivity to heavy metals chiefly mercury means metal-chelating or sequestering compounds must be added to soil contaminated with both acrylamide and heavy metals.
References
Mottram, DS, Wedzicha BL, Dobson AT. Acrylamide is formed in the Maillard reaction. Nature. 2002;419:448-9.
Shukor MY, Gusmanizar N, Azmi NA, Hamid M, Ramli J, Shamaan NA, et al. Isolation and characterization of an acrylamide-degrading Bacillus cereus. J Enviromental Biol. 2009;30(1):57-64.
Sega GA, Valdivia Alcota RP, Tancongco CP, Brimer PA. Acrylamide binding to the DNA and protamine of spermiogenic stages in the mouse and its relationship to genetic damage. Mutat Res Mutagen Relat Subj. 1989 Aug 1;216(4):221-30.
Spencer P, Schaumburg HH. Nervous system degeneration produced by acrylamide monomer. Environ Health Perspect. 1975 Jun 1;11:129-33.
Eikmann T, Herr C. How dangerous is actually acrylamide exposure for the population. Umweltmed Forsch Prax. 2002;7(6):307-8.
Pruser KN, Flynn NE. Acrylamide in health and disease. Front Biosci - Sch. 2011;3 S(1):41-51.
Pennisi M, Malaguarnera G, Puglisi V, Vinciguerra L, Vacante M, Malaguarnera M. Neurotoxicity of acrylamide in exposed workers. Int J Environ Res Public Health. 2013;10(9):3843-54.
Hagmar L, Törnqvist M, Nordander C, Rosén I, Bruze M, Kautiainen A, et al. Health effects of occupational exposure to acrylamide using hemoglobin adducts as biomarkers of internal dose. Scand J Work Environ Health. 2001;27(4):219-26.
Igisu H, Goto I, Kawamura Y, Kato M, Izumi K. Acrylamide encephaloneuropathy due to well water pollution. J Neurol Neurosurg Psychiatry. 1975;38(6):581-4.
Wampler DA, Ensign SA. Photoheterotrophic metabolism of acrylamide by a newly isolated strain of Rhodopseudomonas palustris. Appl Environ Microbiol. 2005;71(10):5850-7.
Buranasilp K, Charoenpanich J. Biodegradation of acrylamide by Enterobacter aerogenes isolated from wastewater in Thailand. J Environ Sci. 2011;23(3):396-403.
Charoenpanich J, Tani A. Proteome analysis of acrylamide-induced proteins in a novel acrylamide-degrader Enterobacter aerogenes by 2D electrophoresis and MALDI-TOF-MS. Chiang Mai Univ J Nat Sci. 2014;13(1):11-22.
Gusmanizar N, Shukor Y, Ramli J, Syed MA. Isolation and characterization of an acrylamide-degrading Burkholderia sp. strain DR.Y27. J Ris Kim. 2015 Feb 11;2(1):34.
Yu F, Fu R, Xie Y, Chen W. Isolation and characterization of polyacrylamide-degrading bacteria from dewatered sludge. Int J Environ Res Public Health. 2015;12(4):4214-30.
Bedade DK, Singhal RS. Biodegradation of acrylamide by a novel isolate, Cupriavidus oxalaticus ICTDB921: Identification and characterization of the acrylamidase produced. Bioresour Technol. 2018 Aug 1;261:122-32.
Aisami A, Gusmanizar N. Characterization of an acrylamide-degrading bacterium isolated from hydrocarbon sludge. Bioremediation Sci Technol Res. 2019 Dec 28;7(2):15-9.
Othman AR, Rahim MBHA. Modelling the Growth Inhibition Kinetics of Rhodotorula sp. strain MBH23 (KCTC 11960BP) on Acrylamide. Bioremediation Sci Technol Res. 2019 Dec 28;7(2):20-5.
Rusnam, Gusmanizar N. An Acrylamide-degrading Bacterial Consortium Isolated from Volcanic Soil. J Biochem Microbiol Biotechnol. 2021 Dec 31;9(2):19-24.
Rusnam, Gusmanizar N. Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil. J Environ Bioremediation Toxicol. 2022 Aug 5;5(1):32-7.
Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey's Manual of Determinative Bacteriology. 9th ed. Lippincott Williams & Wilkins; 1994.
Costin S, Ionut S. ABIS online - bacterial identification software, http://www.tgw1916.net/bacteria_logare.html, database version: Bacillus 022012-2.10, accessed on Mar 2015. 2015.
Shah M. Microbial Degradation of Acrylamide by Enterobacter spp. Am J Water Resour. 2014 Nov 2;2:134-40.
Bao M, Chen Q, Li Y, Jiang G. Biodegradation of partially hydrolyzed polyacrylamide by bacteria isolated from production water after polymer flooding in an oil field. J Hazard Mater. 2010 Dec 15;184(1):105-10.
Li W, Liu R, Liang F, Yang Z, Shi M. Study on the characteristics of a strain of polyacrylamide-degrading bacteria. Acta Sci Circumstantiae. 2004 Jan 1;24(6):1116-21.
Nakamiya K, Kinoshita S. Isolation of polyacrylamide-degrading bacteria. J Ferment Bioeng. 1995 Jan 1;80(4):418-20.
Jonston JJ, Borden RC, Barlaz MA. Anaerobic biodegradation of alkylbenzenes and trichloroethylene in aquifer sediment down gradient of a sanitary landfill. J Contam Hydrol. 1996;23(4):263-83.
Shimamura Y, Yui T, Horiike H, Masuda S. Toxicity of combined exposure to acrylamide and Staphylococcus aureus. Toxicol Rep. 2022 Jan 1;9:876-82.
Kulkarni NH, Muley AB, Bedade DK, Singhal RS. Cross-linked enzyme aggregates of arylamidase from Cupriavidus oxalaticus ICTDB921: process optimization, characterization, and application for mitigation of acrylamide in industrial wastewater. Bioprocess Biosyst Eng [Internet]. 2019; Available from: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85075085765&doi=10.1007%2fs00449-019-02240-4&partnerID=40&md5=12e064000a11176469878181f8642894
Lakshmikandan M, Sivaraman K, Elaiya Raja S, Vasanthakumar P, Rajesh RP, Sowparthani K, et al. Biodegradation of acrylamide by acrylamidase from Stenotrophomonas acidaminiphila MSU12 and analysis of degradation products by MALDI-TOF and HPLC. Int Biodeterior Biodegrad. 2014;94:214-21.
Chandrashekar V, Chandrashekar C, Shivakumar R, Bhattacharya S, Das A, Gouda B, et al. Assessment of acrylamide degradation potential of Pseudomonas aeruginosa BAC-6 isolated from industrial effluent. Appl Biochem Biotechnol. 2014;173(5):1135-44.
Emmanuel Joshua Jebasingh S, Lakshmikandan M, Rajesh RP, Raja P. Biodegradation of acrylamide and purification of acrylamidase from newly isolated bacterium Moraxella osloensis MSU11. Int Biodeterior Biodegrad. 2013;85:120-5.
Ansede JH, Pellechia PJ, Yoch DC. Metabolism of acrylate to beta-hydroxypropionate and its role in dimethylsulfoniopropionate lyase induction by a salt marsh sediment bacterium, Alcaligenes faecalis M3A. Appl Environ Microbiol. 1999 Nov;65(11):5075-81.
Baek SH, Kim KH, Yin CR, Jeon CO, Im WT, Kim KK, et al. Isolation and characterization of bacteria capable of degrading phenol and reducing nitrate under low-oxygen conditions. Curr Microbiol. 2003;47(6):462-6.
Egorova K, Trauthwein H, Verseck S. Purification and properties of an enantioselective and thermoactive amidase from the thermophilic actinomycete Pseudonocardia thermophila. Appl Microbiol Biotechnol. 2004;38-45.
Rahim MBH, Syed MA, Shukor MY. Isolation and characterization of an acrylamide-degrading yeast Rhodotorula sp . strain MBH23 KCTC 11960BP. J Basic Microbiol. 2012;52(5):573-81.
Rogacheva SM, Ignatov OV. The Respiratory Activity of Rhodococcus rhodochrous M8 Cells Producing Nitrile-Hydrolyzing Enzymes. Appl Biochem Microbiol. 2001;37(3):282-6.
Shukor MY, Ahmad SA, Nadzir MMM, Abdullah MP, Shamaan NA, Syed MA. Molybdate reduction by Pseudomonas sp . strain DRY2. J Appl Microbiol. 2010;108:2050-8.
Wampler DA, Ensign SA. Photoheterotrophic Metabolism of Acrylamide by a Newly Isolated Strain of Rhodopseudomonas palustris. Appl Environ Microbiol. 2005 Oct;71(10):5850-7.
Shen S min, Wan T jou, Hwang H yuan. Biocatalysis and Agricultural Biotechnology Enhancement of degradation of acrylamide coupled with salad oil by Pseudomonas aeruginosa DS-4 using incubation periods. Biocatal Agric Biotechnol. 2012;1(2):110-4.
Wakaizumi M, Yamamoto H, Fujimoto N, Ozeki K. Acrylamide degradation by filamentous fungi used in food and beverage industries. J Biosci Bioeng. 2009;108(5):391-3.
Cha M, Chambliss GH. Characterization of Acrylamidase Isolated from a Newly Isolated Acrylamide-Utilizing Bacterium , Ralstonia eutropha AUM-01. Curr Microbiol. 2011;671-8.
Shukor MY, Gusmanizar N, Ramli J, Shamaan NA, Maccormack WP, Syed MA. Isolation and characterization of an acrylamide-degrading Antarctic bacterium. J Enviromental Biol. 2009;30(1):107-12.
Bedade DK, Muley AB, Singhal RS. Magnetic cross-linked enzyme aggregates of acrylamidase from Cupriavidus oxalaticus ICTDB921 for biodegradation of acrylamide from industrial waste water. Bioresour Technol. 2019;272:137-45.
Bedade DK, Singhal RS. Isolation and Characterization of Acrylamidase from Arthrobacter sp. DBV1 and Its Ability to Biodegrade Acrylamide. Appl Biochem Biotechnol. 2017;182(2):570-85.
Syed MA, Ahmad SA, Kusnin N, Shukor MYA. Purification and characterization of amidase from acrylamide-degrading bacterium Burkholderia sp. strain DR.Y27. Afr J Biotechnol. 2012;11(2):329-36.
Cha M, Chambliss GH. Characterization of acrylamidase isolated from a newly isolated acrylamide-utilizing bacterium, Ralstonia eutropha AUM-01. Curr Microbiol. 2011;62(2):671-8.
Rahman MFA, Yasid NA, Ahmad SA, Shamaan NA, Shukor MY. Characterization of molybdenum-reduction by an acrylamide-degrading Antarctic bacterium. In 10-3 Midori-cho, Tachikawa, Tokyo, Japan: National Institute of Polar Research (NIPR); 2018. Available from: http://id.nii.ac.jp/1291/00015258/
Amor L, Kennes C, Veiga MC. Kinetics of inhibition in the biodegradation of monoaromatic hydrocarbons in presence of heavy metals. Bioresour Technol. 2001 Jun 1;78(2):181-5.
Gopinath KP, Kathiravan MN, Srinivasan R, Sankaranarayanan S. Evaluation and elimination of inhibitory effects of salts and heavy metal ions on biodegradation of Congo red by Pseudomonas sp. mutant. Bioresour Technol. 2011;102(4):3687-93.
Roane TM, Josephson KL, Pepper IL. Dual-Bioaugmentation Strategy To Enhance Remediation of Cocontaminated Soil. Appl Environ Microbiol. 2001 Jul;67(7):3208-15.
Hettiarachchi GM, Pierzynski GM, Ransom MD. In situ stabilization of soil lead using phosphorus and manganese oxide. Environ Sci Technol. 2000;34(21):4614-9.
Babich H, Stotzky G. Effect of Cadmium on Fungi and on Interactions Between Fungi and Bacteria in Soil: Influence of Clay Minerals and pH. Appl Environ Microbiol. 1977 May;33(5):1059-66.
Kamel Z. Toxicity of cadmium to twoStreptomyces species as affected by clay minerals. Plant Soil. 1986 Jun 1;93(2):195-203.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Bioremediation Science and Technology Research
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
