Effect of Growth Media and pH on Microalgal Biomass of Chlorella vulgaris for Biodiesel Production

Authors

  • Baha’uddeen Sa’id Adam Department of Plant Biology, Faculty of Life Sciences, Bayero University, P.M.B. 3011, Kano, Nigeria.
  • Bashir Mohammed Abubakar Department of Biological Sciences, Faculty of Science, Bauchi State University, P.M.B. 65, Gadau, Nigeria.
  • L. Garba Department of Microbiology, Faculty of Science, Gombe State University, P.M.B. 127 Gombe, Nigeria.
  • Ismail Hassan Department of Biological Sciences, Faculty of Science, Bauchi State University, P.M.B. 65, Gadau, Nigeria.

DOI:

https://doi.org/10.54987/bstr.v10i1.684

Keywords:

Algae, Growth Media, Biomass, Chlorella vulgaris, Biodiesel

Abstract

The increased industrialization and overuse of natural resources for energy, such as fossil fuels, have led to the energy crises and environmental issues that plague the world in the twenty-first century. The production of biodiesel from algae has recently gained attention as a potentially useful alternative fuel that is both environmentally friendly and easy to obtain. In this work, the effects of various pH levels on algae biomass and oil production from Chlorella Vulgaris were studied. The growth of the biomass concentration was monitored using a spectrophotometer. The biomass of C. vulgaris obtained from the test was subjected to oil extraction using the chemical solvent method. Out of the five media compositions tested (MBG-11, BG-11, BBM, M8 and N8), MBG-11 recorded the highest biomass concentration at pH 8 (0.6 mg/L/D) and N8 recorded the least biomass concentration at pH 6 (0.49 mg/L/D). The highest percentage of oil was extracted from the C. vulgaris in BBM at pH 6 (31.22%) while the lowest oil was recorded in M8 at pH 8 (14.75%). In conclusion, the best medium for C. vulgaris biomass production was MBG-11 medium while the best medium for oil Production from this microalga was Bold Basal Medium (BBM).

References

Marwa GS, Noura SD, Muhammad SK, Mohamed SZ, Laila M, Magdy El-Bana, et al. High-Throughput Screening of Chlorella vulgaris Growth Kinetics inside a Droplet-Based Microfluidic Device under Irradiance and Nitrate Stress Conditions. Biomolecules. 2019; 9(7): 276.

Bharathiraja B, Chakravarthy M, Kumar RR, Yuvaraj D, Jayamuthunagai J, Kumar RP et al. Palani S. Biodiesel production using chemical and biological methods-A review of process, catalyst, acyl acceptor, source and process variables. Renew and Sustain Energy Rev. 2014;38:368-82.

González?Fernández C, Sialve B, Bernet N, Steyer JP. Impact of microalgae characteristics on their conversion to biofuel. Part I: Focus on cultivation and biofuel production. Biofuel Bioprod Biorefin. 2012;6(1):105-13.

Maadane A, Merghoub N, Mernissi NE, Ainane T, Amzazi S, Bakri IW. Antimicrobial activity of marine microalgae isolated from Moroccan coastlines. J Microbiol Biotechnol Food Sci. 2021;2021:1257-60.

Saad MG, Dosoky NS, Khan MS, Zoromba MS, Mekki L, El-Bana M et al.. High-throughput screening of Chlorella vulgaris growth kinetics inside a droplet-based microfluidic device under irradiance and nitrate stress conditions. Biomolecules. 2019;9(7):276.

Raheem A, Prinsen P, Vuppaladadiyam AK, Zhao M, Luque R. A review on sustainable microalgae based biofuel and bioenergy production: Recent developments. J Cleaner Prod. 2018;181:42-59.

Priyadarshani I, Rath B. Commercial and industrial applications of micro algae-A review. J Algal Biomass Util. 2012;3(4):89-100.

Lv JM, Cheng LH, Xu XH, Zhang L, Chen HL. Enhanced lipid production of Chlorella vulgaris by adjustment of cultivation conditions. Bioresour Technol. 2010;101(17):6797-804.

Zheng H, Yin J, Gao Z, Huang H, Ji X, Dou C. Disruption of Chlorella vulgaris cells for the release of biodiesel-producing lipids: a comparison of grinding, ultrasonication, bead milling, enzymatic lysis, and microwaves. Appl biochem Biotechnol. 2011;164(7):1215-24.

Wong Y, Ho YH, Ho KC, Leung HM, Yung KK. Growth medium screening for Chlorella vulgaris growth and lipid production. J Aquac Mar Biol. 2017;6(1):00143.

Nichols HW, Bold HC. Trichosarcina polymorpha gen. et sp. nov. J Phycol. 1965;1(1):34-8.

Imamoglu E, Sukan FV, Dalay MC. Effect of different culture media and light intensities on growth of Haematococcus pluvialis. Int J Natural Eng Sci. 2007;1(3).

Crofcheck CL, Monstross M, Xinyi E, Shea AP, Crocker M, Andrews R. Influence of media composition on the growth rate of Chlorella vulgaris and Scenedesmus acutus utilized for CO2 mitigation. J ASABE. 2012 (p. 1).

Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY. Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Microbiology. 1979;111(1):1-61.

Abdel-Razek MA, Abozeid AM, Eltholth MM, Abouelenien FA, El-Midany SA, Moustafa NY et al. Bioremediation of a pesticide and selected heavy metals in wastewater from various sources using a consortium of microalgae and cyanobacteria. Slov Vet. 2019;56(22):61 73.

Choi HJ. Effect of eggshells for the harvesting of microalgae species. Biotechnol Biotechnol Equip. 2015;29(4):666-72.

Rizwan UB, Abeera M, Khadim A, Sehar A, Sadam H, Mazhar M et al. Extraction of oil from algae for biodiesel production, from Quetta, Pakistan. Mater Sci Eng. 2018; 414: 1S

Folch J, Lees M., Stanley G.H. S. A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem. 1957; 226(1): 497-509.

Zhu L. Microalgal culture strategies for biofuel production: a review. Biofuel Bioprod Biorefin. 2015;9(6):801-14.

Chen H, Zhou D, Luo G, Zhang S, Chen J. Macroalgae for biofuels production: Progress and perspectives. Renew Sustain Energy Rev. 2015; 47:427-37.

Arun J, Shreekanth SJ, Sahana R, Raghavi MS, Gopinath KP, Gnanaprakash D. Studies on influence of process parameters on hydrothermal catalytic liquefaction of microalgae (Chlorella vulgaris) biomass grown in wastewater. Bioresour Technol. 2017; 244:963-8.

Sharma AK, Sahoo PK, Singhal S, Patel A. Impact of various media and organic carbon sources on biofuel production potential from Chlorella spp. Biotech. 2016;6(2):1-2.

Sharma R, Singh GP, Sharma VK. Comparison of different media formulations on growth, morphology and chlorophyll content of green alga, Chlorella vulgaris. Int J Pharm Biol Sci. 2011;2(2):B509-16.

Li X, Yu Y, Jin M, Hong Q, Chen A and Yang C. Protein digestibility of enzymatic hydrolysis feather meal in vitro and its application in growing Pigs. Chinese J Anim Nutr. 2012;48(15):33-36.

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Published

2022-07-31

How to Cite

Adam, B. S. ., Abubakar, B. M., Garba, L., & Hassan, I. . (2022). Effect of Growth Media and pH on Microalgal Biomass of Chlorella vulgaris for Biodiesel Production. Bioremediation Science and Technology Research (e-ISSN 2289-5892), 10(1), 22–25. https://doi.org/10.54987/bstr.v10i1.684

Issue

Vol. 10 No. 1 (2022)

Section

Articles

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