Isolation and Characterization of Biosurfactant-producing Alcaligenes sp. YLA11 and its Diesel Degradation Potentials

Authors

  • Abdulrahman Abdulhamid Arabo Department of Biochemistry, Bayero University, PMB 3011, Gwarzo Road Kano, Kano, Nigeria.
  • Raji Arabi Bamanga Department of Biotechnology, Modibbo Adama University Yola, Nigeria
  • Mujiburrahman Fadilu Department of Biochemistry and Forensic Science, Nigeria Police Academy, Wudil, Kano State, Nigeria.
  • Musa Abubakar Department of Biological Sciences, Al-Qalam University Katsina, Katsina State, Nigeria.
  • Fatima Abdullahi Shehu Department of Biological Sciences, Al-Qalam University Katsina, Katsina State, Nigeria.
  • Hafeez Muhammad Yakasai Department of Biochemistry, Bayero University, PMB 3011, Gwarzo Road Kano, Kano, Nigeria.
  • Nasiru Abdullahi

DOI:

https://doi.org/10.54987/bstr.v9i2.619

Keywords:

Diesel oil, Biosurfactant, Alkanes, Alcaligenes sp., Biodegradation

Abstract

This study aimed to isolate and identify biosurfactant producing and diesel alkanes degrading bacteria. For this reason, bacteria isolated from the diesel contaminated site were screened for their potential to produce biosurfactants and degrade diesel alkanes. Primary selection of diesel degraders was carried out by using conventional enrichment culture technique where 12 bacterial strains were isolated based on their ability to grow on minimal media supplemented with diesel as sole carbon source, which was followed by qualitative screening methods for potential biosurfactant production. Isolate B11 was the only candidate that shows positive signs for drop collapse, foaming, haemolytic test, oil displacement of more than 22 ± 0.05 mm, and emulsification (E24) of 14 ± 0.30%. The effect of various culture parameters (incubation time, diesel concentration, nitrogen source, pH and temperature) on biodegradation of diesel was evaluated. The optimum incubation time was confirmed to be 120 days for isolates B11, the optimum PH was confirmed as 8.0 for the isolate, Similarly, the optimum temperature was confirmed as 35oC. In addition, diesel oil was used as the sole carbon source for the isolates. The favourable diesel concentration was 12.5 % (v/v) for the isolate. The isolate has shown degradative ability towards Tridecane (C13), dodecane, 2, 6, 10-trimethyl- (C15), Tetradecane (C14), 2,6,10-Trimethyltridecane (C16), Pentadecane (C15). It degraded between 0.27% - 9.65% individual diesel oil alkanes. The strain has exhibited the potential of degrading diesel oil n-alkanes and was identified as Alcaligenes species strain B11 (MZ027604) using the 16S rRNA sequencing.

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Published

2021-12-31

How to Cite

Arabo, A. A., Bamanga, R. A., Fadilu, M., Abubakar, M., Shehu, F. A., Yakasai, H. M., & Abdullahi, N. (2021). Isolation and Characterization of Biosurfactant-producing Alcaligenes sp. YLA11 and its Diesel Degradation Potentials. Bioremediation Science and Technology Research, 9(2), 7–12. https://doi.org/10.54987/bstr.v9i2.619

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