Decolourisation of Reactive Red 120 by a Heavy Metal-tolerant Bacterium Isolated from Juru River, Malaysia

Authors

  • Motharasan Manogaran Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Baarathyee Manogaran Department of Science & Biotechnology, Faculty of Engineering & Life Sciences, Universiti Selangor, Jalan Timur Tambahan, 45600 Bestari Jaya, Selangor
  • Ahmad Razi Othman Department of Chemical and Process Engineering, Universiti Kebangsaan Malaysia,43600 UKM, Bangi, Selangor, Malaysia.
  • Baskaran Gunasekaran Faculty Of Applied Science, UCSI University, 56000 Kuala Lumpur, Selangor, Malaysia.
  • Mohd Yunus Abd Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v8i1.512

Keywords:

Pseudomonas, reactive Red 120, heavy metal, decolourisation, bioremediation

Abstract

Application of dyes is prevalent in industries involved in textile and food manufacturing. Effluent discharge from these industries to neighbouring water bodies cause significant health concerns due to dye toxicity. To date, only very few bacteria are isolated with the ability to completely assimilate dyes. The main objective of this study is to isolate bacteria(s) with the ability to utilise reactive red dye 120. Local strains were isolated from contaminated sites in Northern Malaysia. Based on 16S rDNA gene sequence analysis, the best strain was identified as Pseudomonas sp. strain DRY011. Optimum RR120 decolourisation was observed at 200 ppm with 71.07% removal rate within 5 days and able to tolerate up to 500 ppm. The effect of heavy metals (silver, arsenic, cadmium, chromium, copper, mercury, lead and zinc) were investigated. Mercury, 1 ppm had the highest inhibition effect, followed by zinc and silver, with decolourisation of 12 % and 14.3 % respectively. Chromium had the least effect with 55.6% decolourisation and bacterial growth of 11.5 CFU/mL. The rest of the heavy metal had the least effect on the decolourisation rate. As a conclusion, the finding of microbial degrader able to utilise dye will become crucial bioremediation key in controlling the level of dye wastes in water bodies.

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Published

2020-07-31

How to Cite

Manogaran, M., Manogaran, B., Othman, A. R., Gunasekaran, B., & Shukor, M. Y. A. (2020). Decolourisation of Reactive Red 120 by a Heavy Metal-tolerant Bacterium Isolated from Juru River, Malaysia. Bioremediation Science and Technology Research (2289-5892), 8(1), 23–26. https://doi.org/10.54987/bstr.v8i1.512

Issue

Vol. 8 No. 1 (2020)

Section

Articles

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