Allochthonous Diesel Biodegradation by Bacillus sp. MO1 Isolated from Diesel-contaminated Soil

Authors

  • Mohd Syahmi Ramzi Salihan Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Syahir Habib Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Farrah Aini Dahalan School of Environmental Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia.
  • Nor Ayshah Alia Ali Hassan Soil, Water, and Fertilizer Research Centre, Malaysian Agricultural Research and Development Institute, Persiaran MARDI-UPM, 43400 Serdang, Selangor.
  • Mohd Arif Syed Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Shafinaz Abd. Gani Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Mohd Yunus Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/bstr.v7i1.459

Keywords:

Allochthonous; isolation; characterization; diesel-degrading; Bacillus sp.

Abstract

Ten bacterial isolates were successfully isolated from pristine and contaminated areas in Malaysia. There was no lag phase observed signifying each of these isolates was able to quickly induce cellular machinery for diesel assimilation. The best isolate was tentatively identified as Bacillus sp. strain MO1 based on carbon utilisation profiles using Biolog GP plates and partial 16S rDNA matching. Strain MO1 grew optimally at 37 oC on 4% (v/v) diesel, pH 7.5 and 0.9% (w/v) KNO3. Diesel components were proven to be completely eliminated from the reduction in the hydrocarbon peaks monitored by gas chromatographic analysis after 6 days of incubation. The bacterium was found to be a better remediation agent than a commercial bioremediation product. All of these characteristics suggest that this bacterium is the suitable bacterium for bioremediation of diesel spills and pollution in the tropics.

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Published

2019-07-31

How to Cite

Salihan, M. S. R., Habib, S., Dahalan, F. A., Ali Hassan, N. A. A., Syed, M. A., Gani, S. A., & Shukor, M. Y. (2019). Allochthonous Diesel Biodegradation by Bacillus sp. MO1 Isolated from Diesel-contaminated Soil. Bioremediation Science and Technology Research, 7(1), 18–23. https://doi.org/10.54987/bstr.v7i1.459

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Articles