Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium

Authors

  • Noor Suffiah Md. Zin Zin Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Siti Nadzirah Padrilah Padrilah Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Fadhil Abd Rahman Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Han Koh Sim Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Ariff Khalid Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
  • Mohd Yunus Shukor Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v4i2.373

Keywords:

2,4-dinitrophenol; 2,4-dinitrophenol-degrading; Bacterium; bioremediation

Abstract

2,4-dinitrophenol (2,4-DNP) is utilized in the production of wood preservatives, dyes, and also as a pesticide. Human acute (short-term) exposure to 2,4-DNP in humans by means of oral exposure are nausea or vomiting, sweating, headaches, dizziness, and weight reduction. Thus, the removal of this compound is highly sought. A 2,4-DNP-degrading bacterium (isolate 1) was isolated from a sample soil from Terengganu. This bacterium (isolate 1) was characterized as a rod Gram positive, non-sporulated, and non-motile bacterium. The bacterium is oxidase negative and had catalase positive activity and was able to grow aerobically on 2,4-dinitrophenol as the sole carbon source. This bacterium showed maximal growth on 2,4-DNP at the temperature optimum of 30 oC, pH 5.0 and was tolerant to 2,4-DNP concentration of up to 0.5 mM (0.092 g/L). This bacterium prefers to use urea as the nitrogen source in addition to yeast extract for mineral source and vitamin precursors.

 

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Published

2016-12-31

How to Cite

Zin, N. S. M. Z., Padrilah, S. N. P., Rahman, M. F. A., Sim, H. K., Khalid, A., & Shukor, M. Y. (2016). Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium. Bioremediation Science and Technology Research, 4(2), 28–33. https://doi.org/10.54987/bstr.v4i2.373

Issue

Vol. 4 No. 2 (2016)

Section

Articles

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