Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna

Authors

  • Lubna Kamil Abdulhussein Abo-Shakeer Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Hafeez Yakasai Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Fadhil Abd Rahman Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Nurlizah Abu Bakar nstitute of Ocean and Earth Sciences, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
  • Mohd Arif Syed Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Norhani Abdullah Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia 43400 UPM Serdang Selangor Darul Ehsan, Malaysia.
  • Ahmad Razi Othman Department of Chemical Engineering and Process, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v4i1.366

Keywords:

Molybdenum; Mo-reducing enzyme; ammonium sulfate fractionation; gel filtration chromatography; SDS-PAGE

Abstract

Molybdenum is an emerging pollutant. Bioremediation of this heavy metal is possible by the mediation of Mo-reducing bacteria. These bacteria contain the Mo-reducing enzymes that can conver toxic soluble molybdenum into molybdenum blue; a less soluble and less toxic form of the metal. To date only the enzyme has been purified from only one bacterium. The aim of this study is to purify the Mo-reducing enzyme from a previously isolated Mo-reducing bacterium Bacillus pumilus strain Lbna using ammonium sulphate fractionation followed by ion exchange and then gel filtration. Two clear bands were obtained after the gel filtration step with molecular weights of 70 and 100 kDa. This indicates that further additional purification methods need to be used to get a purified fraction. Hence, additional steps of chromatography such as hydroxyapatite or chromatofocusing techniques can be applied in the future.

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Published

2016-07-31

How to Cite

Abo-Shakeer, L. K. A., Yakasai, M. H., Rahman, M. F. A., Bakar, N. A., Syed, M. A., Abdullah, N., & Othman, A. R. (2016). Partial purification of the molybdenum-reducing enzyme from Bacillus pumilus strain Lbna. Bioremediation Science and Technology Research (e-ISSN 2289-5892), 4(1), 14–17. https://doi.org/10.54987/bstr.v4i1.366

Issue

Vol. 4 No. 1 (2016)

Section

Articles

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