Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12

Authors

  • Siti Nur Khadijah Jamulidin Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Motharasan Manogaran Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Hafeez Yakasai Department of Biochemistry, Faculty of Basic Medical Science, College of Health Sciences, Bayero University Kano, P.M.B 3011, Nigeria.
  • Mohd Fadhil Abd Rahman Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Yunus Shukor Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v4i1.363

Keywords:

Molybdenum-reducing bacterium; Michaelis-Menten constants; NADH; Burkholderia vietnamiensis; glyphosate-degrading bacterium

Abstract

In this study, a novel glyphosate-degrading shows the ability to reduce molybdenum to molybdenum blue. The enzyme from this bacterium was partially purified and partially characterized to ascertain whether the Mo-reducing enzyme from this bacterium shows better or lower efficiency in reducing molybdenum compared to other Mo-reducing bacterium that only exhibits a single biotransformation activity. The enzyme was partially purified using ammonium sulphate fractionation. The Vmax for the electron donating substrate or NADH was at 1.905 nmole Mo blue/min while the Km was 6.146 mM. The regression coefficient was 0.98. Comparative assessment with the previously characterized Mo-reducing enzyme from various bacteria showed that the Mo-reducing enzyme from Burkholderia vietnamiensis strain AQ5-12 showed a lower enzyme activity.

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Published

2016-07-31

How to Cite

Jamulidin, S. N. K., Manogaran, M., Yakasai, M. H., Rahman, M. F. A., & Shukor, M. Y. (2016). Partial purification and characterization of the molybdenum-reducing enzyme from the glyphosate-degrading Burkholderia vietnamiensis strain AQ5-12. Bioremediation Science and Technology Research (e-ISSN 2289-5892), 4(1), 7–9. https://doi.org/10.54987/bstr.v4i1.363

Issue

Vol. 4 No. 1 (2016)

Section

Articles

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