Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP

Authors

  • Syahir Habib Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Wan Lutfi Wan Johari Department of Environmental Science, Faculty of Environment, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Mohd Yunus Shukor Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Nur Adeela Yasid Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v5i2.358

Keywords:

petroleum hydrocarbons ; hydrocarbon-degrading isolates; redox indicator; 2,6-dichlorophenolindophenol

Abstract

Petroleum hydrocarbons remain as the major contaminants that could be found across the world. Remediation approach through the utilisation of microbes as the bioremediation means is widely recognised due to their outstanding values. As a result, scientific reports on the isolation and identification of new hydrocarbon-degrading strains were on the rise. Colourimetric-based assays are one of the fastest methods to identify the capability of hydrocarbon-degrading strains in both qualitative and quantitative assessment. In this study, the hydrocarbon-degrading potential of nine bacterial isolates was observed via 2,6-dichlorophenolindophenol (DCPIP) test. Two potent diesel-utilising isolates show a distinctive tendency to utilise aromatic (ADL15) and aliphatic (ADL36) hydrocarbons. Both isolates prove to be a good candidate for bioremediation of wide range of petroleum hydrocarbon components.

Downloads

Published

2017-12-31

How to Cite

Habib, S., Johari, W. L. W., Shukor, M. Y., & Yasid, N. A. (2017). Screening of hydrocarbon-degrading bacterial isolates using the redox application of 2,6-DCPIP. Bioremediation Science and Technology Research, 5(2), 13–16. https://doi.org/10.54987/bstr.v5i2.358

Issue

Vol. 5 No. 2 (2017)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).