Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus

Authors

  • Ain Farihah Zulkifli Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Lik Gin Tham Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Natarajan Perumal Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Khalizan Sabullah Fakulti Sains dan Sumber Alam, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia
  • Azzreena Mohamad Azzeme Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Yunus Shukor Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Noor Azmi Shaharuddin Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v5i1.351

Keywords:

Channa striatus; acetylcholinesterase; heavy metals; inhibitive assay

Abstract

Acetylcholinesterase (AChE) is usually used as an inhibitive assay for insecticides. A lesser known property of AChE is its inhibition by heavy metals. In this work we evaluate an AChE from brains of striped snakehead (Channa striatus) wastes from aquaculture industry as an inhibitive assay for heavy metals. We discovered that the AChE was inhibited almost completely by Hg2+, Ag2+ and Cu2+ during an initial screening. When tested at various concentrations, the heavy metals exhibited exponential decay type inhibition curves. The calculated IC50 for the heavy metals Hg2+, Ag2+, Pb2+, Cu2+ and Cr6+ were 0.08432, 0.1008, 0.1255, 0.0871, and 0.1771, respectively. The IC50 for these heavy metals are comparable and some are lower than the IC50 values from the cholinesterases from previously studied fish. The assay can be carried out in less than 30 minutes at ambient temperature.

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Published

2017-07-31

How to Cite

Zulkifli, A. F., Tham, L. G., Perumal, N., Sabullah, M. K., Azzeme, A. M., Shukor, M. Y., & Shaharuddin, N. A. (2017). Assay for heavy metals using an inhibitive assay based on the acetylcholinesterase from Channa striatus. Bioremediation Science and Technology Research (e-ISSN 2289-5892), 5(1), 7–11. https://doi.org/10.54987/bstr.v5i1.351

Issue

Vol. 5 No. 1 (2017)

Section

Articles

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