Observation of the bile canaliculi of <i>Puntius javanicus</i> liver affected by copper
DOI:
https://doi.org/10.54987/bstr.v3i2.298Keywords:
Puntius javanicus, copper, bile canaliculi, Transmission Electron Microscopy, UltrastructureAbstract
Investigation on in vivo effects of copper (Cu) on the ultrastructure of P. Javanicus liver was carried out using transmission electron microscopy (TEM). The addition of sublethal concentration of 5 mg/L of Cu caused abnormalities on the bile canaliculi (BC) including dilation and elongation compared to control and at lower concentrations of copper with a normal round shape form. Findings from this study support an alternative histological assessment of the effects of Cu concentration on P. Javanicus liver.
References
Sabullah MK, Ahmad SA, Shukor MY, Gansau AJ, Syed MA, Sulaiman MR, Shamaan NA. Heavy metal biomarker: Fish behavior, cellular alteration, enzymatic reaction and proteomics approaches. Int Food Res J. 2015;22(2):435-54.
Wei Y, Zhu N, Lavoie M, Wang J, Qian H, Fu Z. Copper toxicity to Phaeodactylum tricornutum: a survey of the sensitivity of various toxicity endpoints at the physiological, biochemical, molecular and structural levels. Biometals. 2014;27(3):527-37.
Islam E, Yang X, He Z, Mahmood Q. Assessing potential dietary toxicity of heavy metals in selected vegetables and food crops. J Zhejiang Univ Sci B. 2007;8(1): 1–13.
Nica DV, Bura M, Gergen I, Harmanescu M, Bordean D. Bioaccumulative and conchological assessment of heavy metal transfer in a soil-plant-snail food chain. Chem Central J. 2012;6:55.
Sabullah MK., Shukor MY, Sulaiman MR, Shamaan NA, Syed MA, Khalid A, Ahmad SA. The effect of copper on the ultrastructure of Puntius javanicus hepatocyte. Aust J Basic Appl Sci. 2014;8(15):245-51.
Liu XJ, Luoa Z, Xiong BX, Liu X, Zhao YH, Hu GF, Lv GJ. Effect of waterborne copper exposure on growth, hepatic enzymatic activities and histology in Synechogobius hasta. Ecotoxicol Environ Saf. 2010;73:1286–91.
Ajani EK, Akpoilih BU. Effect of chronic dietary copper exposure on haematology and histology of common carp (Cyprinus carpio L.). J Appl Sci Environ Manage. 2010;14:39-45.
Deore SV, Wagh SB. Heavy metal induced histopathological alterations in liver of Channa gachua (Ham). J Exp Biol. 2012;3: 35-8.
Younis EM, Abdel-Warith AA, Al-Asgah NA, Ebaid H, Mubarak M. Histological changes in the liver and intestine of Nile Tilapia, Oreochromis niloticus, exposed to sublethal concentrations of cadmium. Pak J Zool. 2013;45(3): 833-41.
Kodiha M, Chu A, Matusiewicz N, Stochaj U. Multiple mechanisms promote the inhibition of classical nuclear import upon exposure to severe oxidative stress. Cell Death Different. 2004;11:862–74.
Sánchez L, Kodiha M, Stochaj U. Monitoring the disruption of nuclear envelopes in interphase cells with GFP-beta-galactosidase. J Biomol Tech. 2005;16:235–238.
Linder MC. The relationship of copper to DNA damage and damage prevention in humans. Mut Res. 2012;733:83– 91.
Roberts EA, Sarkar B. Liver as a key organ in the supply, storage, and excretion of copper. Am J Clin Nut. 2008;88(3):851S-854S.
Azumi N. Copper and liver injury--experimental studies on the dogs with biliary obstruction and copper loading. Hokkaido Igaku Zasshi. 1982;57(3):331-49.
Albores A, Cebrian ME, Garcia-Vargas GG, Connelly JC, Price SC, Hinton RH, Bach PH, Bridges JW. Enhanced arsenite-induced hepatic morphological and biochemical changes in phenobarbital-pretreated rats. Toxicol Pathol. 1996;24(2):172-180.
Narayana K, Al-Bader M. Ultrastructural and DNA damaging effects of lead nitrate in the liver. Exp Toxicol Pathol. 2011;63:43-51.
Jattujan P, Pinlaor S, Charoensuk L, Arunyanart C, Welbat JU, Chaijaroonkhanarak W. Curcumin prevents bile canalicular alterations in the liver of hamsters infected with Opisthorchis viverrini. Korean J Parasitol. 2013;51(6): 695–701.
Tag CG, Sauer-Lehnen S, Weiskirchen S, Borkham-Kamphorst E, Tolba RH, Tacke F, et al. Bile duct ligation in mice: induction of inflammatory liver injury and fibrosis by obstructive cholestasis. J Vis Exp. 2015;96: e52438, doi:10.3791/52438.
Wang K. Molecular mechanisms of hepatic apoptosis. Cell Death Disease. 2014;5:e996
Rhee JS, Yu IT, Kim BM, Jeong CB, Lee KW, Kim MJ, Lee SJ, Park GS, Lee JS. Copper induces apoptotic cell death through reactive oxygen species-triggered oxidative stress in the intertidal copepod Tigriopus japonicus. Aquat Toxicol. 2013;15(132-133):182-9.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).