Isolation, Characterization and Growth Optimization of a Chromate-reducing Bacterium

Authors

  • Nor Ashikin Sopian Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia
  • Mohd Izuan Effendi Halmi Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.
  • Mohd Yunus Abd Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.
  • Mohd Khalizan Sabullah Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.
  • Wan Lutfi Wan Johari Environmental Forensics Research Centre, Faculty of Environmental Studies, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/bstr.v2i2.178

Abstract

The application of chromium in industries has introduced anthropogenic release of chromate
which is very toxic and a soluble environmental contaminant. The contaminated sites and
industrial wastewater containing chromate are treated based on reduction of Cr(VI) to Cr(III)
which is insoluble and less toxic to environment. This can be achieved through utilization of
Cr(VI)-reducing bacteria which serves more economical and environmental-friendly treatment
as compared to conventional chemical treatment method. To fulfill this, a new chromate
reducing bacterium was isolated from an agricultural site. This bacterium was identified as
Aeromonas encheleia using Biolog analysis with 0.603 similarity index. Chromate reduction
capability of the bacterium was optimized using Response Surface Methodology with optimum
conditions occurring at 250 μM Cr(VI), temperature 34 °C and pH 7.5 with 99% reduction was
achieved. The bacterium showed high tolerance in LB media containing 3.0 mM of K2Cr2O7,
but only reduce 1.5 mM Cr(VI) within 24 hours. The presence of metals and metabolic
inhibitors in the media did not give significant inhibitory effect on Cr(VI) removal except Hg,
NaCN and Co with percentage inhibition 6.7, 80.5 and 90.8 respectively. Based on this study,
Aeromonas encheleia could be a good candidate in bioremediation of contaminated site or
industrial wastewater containing Cr(VI).

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Published

2015-01-31

How to Cite

Sopian, N. A., Halmi, M. I. E., Shukor, M. Y. A., Sabullah, M. K., & Johari, W. L. W. (2015). Isolation, Characterization and Growth Optimization of a Chromate-reducing Bacterium. Bioremediation Science and Technology Research (e-ISSN 2289-5892), 2(2), 18–24. https://doi.org/10.54987/bstr.v2i2.178

Issue

Vol. 2 No. 2 (2014)

Section

Articles

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