Comparative Analysis of Amylase Enzyme Produced by Aspergillus niger Using Rice and Maize Bran as Substrates

Authors

  • A. Bukar Department of Microbiology, Faculty of Science, Bayero University Kano, PMB 3011 Gwarzo Road Kano, Nigeria.
  • U. Musa Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.
  • A.B. Yusuf Department of Biochemistry, Faculty of Science, Federal University Gashua, P. M. B 1005 Gashua, Yobe State Nigeria.
  • H.U. Puma Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.
  • I.U. Tawfiq Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.
  • I. Mansur Department of Biological Science University of Kashere, P.M.B. 0182 Gombe, Gombe State, Nigeria.
  • M.M. Ahmad Department of Microbiology, Faculty of Science, Gombe State University, PMB 127, Tudun Wada Gombe, 760001 Gombe State, Nigeria.

DOI:

https://doi.org/10.54987/ajpb.v5i1.823

Keywords:

Aspergillus niger, Miaze bran, Amylase, Soil, Rice bran

Abstract

Amylase is used in the degradation of starch-containing materials into its simpler constituents. This study was aimed at comparing amylase production by Aspergillus niger isolated from different sources on different varieties of rice and maize bran. Different strains of A. niger were isolated from different sources and were identified using cultural, morphological and molecular characteristics. A. niger USGB:1024 isolated from soil obtained from a maize mill and A. niger BGSU:17-00030 isolated from spoiled tomato were used for amylase enzyme production as both gave positive results during screening. Amylase enzyme was produced using 15g of substrates, temperature 30 C, pH 6 and inoculum density of 1 ×106. A. niger USGB:1024 was found to produce an amylase enzyme with the highest activity (3.15±0.1 IU) on Chinese rice bran at fermentation day 5. A. niger BGSU:17-00030 also produced amylase enzyme with the highest activity (1.46±0.01 IU) on Chinese rice bran at 5 days of fermentation. The result has shown that A. niger USGB:1024 produced amylase enzyme with the highest activity than A. niger BGSU:17-00030.

References

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Published

31.07.2023

How to Cite

Bukar, A., Musa, U., Yusuf, A., Puma, H., Tawfiq, I., Mansur, I., & Ahmad, M. (2023). Comparative Analysis of Amylase Enzyme Produced by Aspergillus niger Using Rice and Maize Bran as Substrates. Asian Journal of Plant Biology, 5(1), 21–25. https://doi.org/10.54987/ajpb.v5i1.823

Issue

Vol. 5 No. 1 (2023)

Section

Articles

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