Modelling the Growth of Callus Cultures from Glycine wightii (Wight & Arn.) Verdc.

  • M.S. . Shukor Snoc International Sdn Bhd, Lot 343, Jalan 7/16 Kawasan Perindustrian Nilai 7, Inland Port, 71800, Negeri Sembilan, Malaysia.
  • N.A. Masdor Biotechnology Research Centre, MARDI, P. O. Box 12301, 50774 Kuala Lumpur, Malaysia
  • M.I.E. Halmi Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • S.A. Ahmad Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
Keywords: callus growth curve, mathematical model, Glycine wightii, modified Gompertz model, least square method


One of the most important preliminary investigations of callus attributes is the growth characteristics. Most often than not, callus growth curve is sigmoidal in characteristics. In this work, we model callus growth from Glycine wightii from published literature to acquire essential growth constants. These growth constants are only able to be precisely extracted from mathematical modelling of the growth curves using numerous readily available primary modelsfor example logistic, Gompertz, Richards, Schnute, Baranyi-Roberts, Von Bertalanffy, Buchanan three-phase and more recently Huang models. Statistical tests such as root-mean-square error (RMSE), adjusted coefficient of determination (R2), bias factor (BF), accuracy factor (AF) and corrected AICc (Akaike Information Criterion) were utilized to find the best model. The bestmodel was modified Gompertz. The growth constants obtained obtained such as lag, Ymax, μmax were 3.78 d, 6.07 mg, and 0.318 d-1, respectively, respectively. Growth parameter constants extracted from the modelling exercise will be helpful for additional secondary modelling implicating the consequence of media conditions as well as other factors on the growth of callus from this plant.


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