Identification of Bacteria from Soil of Cameron Highlands

Authors

  • Parveen Devi Pattiram Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Baskaran Gunasekaran Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • Norazah Bt Mohammad Nawawi Faculty of Sciences and Biotechnology, Universiti Selangor, 45 600 Bestari Jaya, Selangor, Malaysia.

DOI:

https://doi.org/10.54987/ajpb.v2i2.187

Keywords:

Bacteria, 16S rDNA, BLAST, Bacillus pumilus

Abstract

The bacteria are a large group of unicellular microorganisms. Bacteria are ubiquitous in every
habitat on Earth for example growing in soil, radioactive waste, water and as well as in organic
matter and the live bodies of plants and animals. They are small cells, found in the environment
as either individual cells or aggregated together as clumps. This study was aimed to identify the
bacteria from soil sample obtained from Cameron Highlands. Eight samples were inoculated on
Nutrient Agar plate. After obtaining pure culture, stock cultures on Nutrient Agar slant and
glycerol stock were prepared and the samples were kept in -20ËšC. Next the samples were
further studied using microscopic observation. From this technique, seven Gram positive
bacteria and one Gram negative bacteria were obtained. The Gram positive rod shaped was
analyzed further using the molecular technique 16S rDNA using pA and pH primers. Then the
sample proceeds to bulk. PCR product of approximately 1kb in size was obtained. The purity
value of the DNA sample obtained was 1.16 A260/280. The sample was sent for sequencing to
NHK Bioscience and Macrogreen (Korea). Only one sample showed positive for 16S rDNA
fragments which were analyzed using Basic Local Alignment Search Tool (BLAST) for
bacterial identification. The data analysis of the sequence shows that the bacterium obtained
was Bacillus pumilus strain.

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Published

30.12.2014

How to Cite

Pattiram, P. D., Gunasekaran, B., & Nawawi, N. B. M. (2014). Identification of Bacteria from Soil of Cameron Highlands. Asian Journal of Plant Biology, 2(2), 39–47. https://doi.org/10.54987/ajpb.v2i2.187

Issue

Vol. 2 No. 2 (2014)

Section

Articles

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