The Application of Plant Proteases from Garlic (Allium sativum) for Biomonitoring of Heavy Metals in the Environment

Authors

  • M.I.E. Halmi Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • N.S.M. Sakeh Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • N.A. Masdor Biotechnology Research Centre, MARDI, P. O. Box 12301, 50774 Kuala Lumpur, Malaysia
  • Baskaran Gunasekaran Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • Helmi Wasoh Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia.
  • M.A. Syed Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • W.L.W. Johari epartment of Environment, Faculty of Environmental Studies, Universiti Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia
  • M.Y. Shukor Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, UPM 43400 Serdang, Selangor, Malaysia

DOI:

https://doi.org/10.54987/ajpb.v2i2.182

Keywords:

garlic (Allium sativum), proteases, iomonitor, heavy metals

Abstract

In this work, a novel source of protease for the bioassay of heavy metals has been developed
using proteases extracted from garlic (Allium sativum). The principle of casein-Coomassie-dye
binding assay has been applied for this purpose. The optimum concentration of enzyme and
substrate were determined at 0.7 mg/mL and 0.175 mg/mL, respectively. The crude proteases of
Allium sativum exhibited optimal activity at pH 6.0, temperature at 40oC and incubation time of
60 minutes. The IC50 values for mercury, copper, cadmium, nickel and chromium were 0.0590
mg/L, 0.6398 mg/L, 1.291 mg/L, 0.9865 mg/L and 1.871 mg/L, respectively. The calculated
limits of quantitation (LOQ) value for mercury, copper, cadmium, nickel and chromium were
0.0188 mg/L, 0.025 mg/L, 0.50 mg/L, 0.10 mg/L and 0.5 mg/L, respectively. The calculated
LOD values for mercury, copper, cadmium, nickel and chromium were 0.0002 mg/L, 0.006
mg/L, 0.05 mg/L, 0.02mg/L and 0.1 mg/L, respectively. The crude proteases extracted from
Allium sativum showed good potential for the development of a rapid, sensitive, and economic
inhibitive assay for the biomonitoring of mercury, copper, cadmium, nickel and chromium in
the environments.

Downloads

Published

30.12.2014

How to Cite

Halmi, M., Sakeh, N., Masdor, N., Gunasekaran, B., Wasoh, H., Syed, M., Johari, W., & Shukor, M. (2014). The Application of Plant Proteases from Garlic (Allium sativum) for Biomonitoring of Heavy Metals in the Environment. Asian Journal of Plant Biology, 2(2), 53–59. https://doi.org/10.54987/ajpb.v2i2.182

Issue

Vol. 2 No. 2 (2014)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).