Characterization of COOH-Fe<sub>3</sub>O<sub>4</sub>/NCC-CTA<sup>+</sup> on screen printed carbon electrode using Field Emission Scanning Electron Microscope and Energy Dispersive X-Ray for DNA biosensor

  • Che Engku Noramalina Che-Engku-Chik Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.
  • Nor Azah Yusof Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.
  • Jaafar Abdullah Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.
  • Siti Sarah Othman Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.
  • Helmi Wasoh Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43300 Serdang, Selangor Darul Ehsan, Malaysia.

Abstract

A novel DNA biosensing platform was designed by the functionalization of iron oxide (Fe3O4)with the carboxylic group via capping agent, mercaptopropionic acid (MPA) and conjugatedwith nanocellulose crystalline (NCC) surface modified with surfactant cetyltrimethylammoniumbromide (CTAB) to assist in the DNA sensing capability. The product of nanocompositescompound was drop-casted on screen printed carbon electrode (SPCE). Characterization by fieldemission scanning electron microscope (FESEM) and energy dispersive X-Ray (EDX)spectroscopy showing that carboxyl functionalized iron oxide (COOH-Fe3O4) can be hybridizedwith NCC-CTA+ via electrostatic interaction.
Published
2017-12-31
How to Cite
CHE-ENGKU-CHIK, Che Engku Noramalina et al. Characterization of COOH-Fe3O4/NCC-CTA+ on screen printed carbon electrode using Field Emission Scanning Electron Microscope and Energy Dispersive X-Ray for DNA biosensor. Journal of Biochemistry, Microbiology and Biotechnology, [S.l.], v. 5, n. 2, p. 19-21, dec. 2017. ISSN 2289-5779. Available at: <http://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/343>. Date accessed: 16 aug. 2018.
Section
Articles