Isolation of molybdenum-reducing bacterium; <i>Serratia</i> sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation
AbstractMolybdenum is reported to be very toxic to ruminants and shows evidence of spermatogenesistoxicity in animals and insects. Hence, its removal is important. In this study, we report on thefirst isolation of molybdenum-reducing bacterium from agricultural soil. The bacterium reduceshexavalent molybdenum (sodium molybdate) to molybdenum blue (Mo-blue); a colloidalproduct, which can be trapped and removed from solution. Phylogenetic analysis resulted in atentative identification of the bacterium as Serratia sp. strain MIE2. The optimum conditions forMo-blue production using the normal one-variable-at-a-time (OVAT) approach were 10 mM ofsodium molybdate, pH 6.0, a temperature of 35°C, ammonium sulphate at 10 g/L as the nitrogensource and sucrose concentrations of between 30-50 g/L as the carbon source and electron donorfor molybdate. Studies on the effects of pesticides and solvents on Mo-blue production showedthat Mo-blue production from whole cells was relatively more affected by these xenobioticscompared to the crude enzyme. Nevertheless, the strain was resistant to most of the xenobioticstested. Based on the strain MIE characteristics, the bacterium will be a suitable candidate for theremediation of aquatic bodies and agricultural soils contaminated with molybdenum.
How to Cite
HALMI, Mohd Izuan Effendi et al. Isolation of molybdenum-reducing bacterium; Serratia sp. strain MIE2 from agriculture soil and its potential use in soil bioremediation. Journal of Biochemistry, Microbiology and Biotechnology, [S.l.], v. 5, n. 2, p. 12-18, dec. 2017. ISSN 2289-5779. Available at: <http://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/342>. Date accessed: 16 aug. 2018.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).