Statistical optimization of hexavalent molybdenum reduction by <i>Serratia</i> sp. strain MIE2 using Central Composite Design (CCD)

  • Nor Farahim Aziz Department of Environmental Science, Faculty of Environment, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Mohd Izuan Effendi Halmi Department of Land Management, Faculty of Agriculture, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Wan Lutfi Wan Johari Department of Environmental Science, Faculty of Environment, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.

Abstract

The conversion of hexavalent molybdenum (Mo (VI)) to Mo-blue is a bioremediation techniquewhich reduces the toxicity of molybdenum to a less toxic form by bacteria. The aim of this studyis to determine the optimum conditions of significant parameters or variables that affect thereduction of Mo (VI) to Mo-blue by the local isolate identified as Serratia sp. strain MIE2.Response Surface Methodology (RSM) was used in this study to optimize the reduction processusing Central Composite Design (CCD) as an optimization matrix. The optimum conditionspredicted by RSM using the desirability function for the reduction process were 20 mMmolybdate concentration, 3.95 mM phosphate, 6.25 pH and 25 g/L glucose and Mo-blueproduction occurred at the absorbance value of 20.5 at 865 nm. The validation of the predictedoptimum points showed the Mo-blue production occurred at the absorbance value of 21.85 witha deviation around 6.6 % from the RSM predicted value.
Published
2017-12-31
How to Cite
AZIZ, Nor Farahim; HALMI, Mohd Izuan Effendi; JOHARI, Wan Lutfi Wan. Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD). Journal of Biochemistry, Microbiology and Biotechnology, [S.l.], v. 5, n. 2, p. 8-11, dec. 2017. ISSN 2289-5779. Available at: <http://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/341>. Date accessed: 21 may 2018.
Section
Articles