Antioxidant activity and phytochemical components of <i>Jatropha curcas</i> Linn. root extract
AbstractJatropha curcas Linn. is a multipurpose plant in the Euphorbiaceae family. Numerous reportshave indicated the antioxidant properties of phenolics and flavonoids present in J. curcas rootmethanolic extract. In the present study, 80% methanolic extract of J. curcas root was preparedand used for extraction of bioactive compounds with five solvents (hexane, chloroform, ethylacetate, n-butanol and water) by liquid-liquid fractionation. The fractions were evaluated fortotal phenolic content (TPC), total flavonoid content (TFC) and antioxidant activities by usingthe 2-2’-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing/antioxidant potential (FRAP) and2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assays. The phytochemicalcompounds present in each fraction were identified by liquid chromatography mass spectrometry(LCMS) analysis. The TPC of ethyl acetate and n-butanol fractions were 34.0 ± 0.02 μg GAE/gDW and 33.1± 0.01 μg GAE /g DW, respectively, while the TFC were 9.2 ± 0.04 μg CE/g DWand 10.1 ± 0.01 μg CE/g DW, respectively. The free radical scavenging activity varied with thesolvents used. Ethyl acetate fraction showed the highest inhibition for DPPH (48.7%) and FRAP(79.6%) assays, while n-butanol fraction showed the highest ABTS radical scavenging activity(94.8%). The LCMS analysis showed the compounds present in the various fractions werephenolic and flavonoid derivatives such as coumaric acid, epigenin, quercetin, leuteolin and pcoumaroylquinicacid. The results showed that TPC, TFC and antioxidant activity for ethylacetate and n-butanol fractions were higher compared to the other solvent fractions.
How to Cite
OSMAN, Shahirah Atiqah; NORHANI ABDULLAH, Norhani; AHMAD, Syahida. Antioxidant activity and phytochemical components of Jatropha curcas Linn. root extract. Journal of Biochemistry, Microbiology and Biotechnology, [S.l.], v. 5, n. 2, p. 2-7, dec. 2017. ISSN 2289-5779. Available at: <http://journal.hibiscuspublisher.com/index.php/JOBIMB/article/view/340>. Date accessed: 16 aug. 2018.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).