Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium

  • Noor Suffiah Md. Zin Zin Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Siti Nadzirah Padrilah Padrilah Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Fadhil Abd Rahman Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Han Koh Sim Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Ariff Khalid Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
  • Mohd Yunus Shukor Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Abstract

2,4-dinitrophenol (2,4-DNP) is utilized in the production of wood preservatives, dyes, and also as a pesticide. Human acute (short-term) exposure to 2,4-DNP in humans by means of oral exposure are nausea or vomiting, sweating, headaches, dizziness, and weight reduction. Thus, the removal of this compound is highly sought. A 2,4-DNP-degrading bacterium (isolate 1) was isolated from a sample soil from Terengganu. This bacterium (isolate 1) was characterized as a rod Gram positive, non-sporulated, and non-motile bacterium. The bacterium is oxidase negative and had catalase positive activity and was able to grow aerobically on 2,4-dinitrophenol as the sole carbon source. This bacterium showed maximal growth on 2,4-DNP at the temperature optimum of 30 oC, pH 5.0 and was tolerant to 2,4-DNP concentration of up to 0.5 mM (0.092 g/L). This bacterium prefers to use urea as the nitrogen source in addition to yeast extract for mineral source and vitamin precursors.
 
Published
2016-12-31
How to Cite
ZIN, Noor Suffiah Md. Zin et al. Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium. Bioremediation Science and Technology Research, [S.l.], v. 4, n. 2, p. 28-33, dec. 2016. ISSN 2289-5892. Available at: <http://journal.hibiscuspublisher.com/index.php/BSTR/article/view/373>. Date accessed: 19 july 2018.
Section
Articles