Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection

  • Umar Abubakar Muhammad Department of Biological Sciences, Faculty of Science, Gombe State University, P.M.B. 027, Gombe, Nigeria.
  • Lik Gin Tham Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Natarajan Perumal Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Hassan Mohd Daud Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Nur Adeela Yasid Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Yunus Shukor Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Abstract

In this work we assess the potential of acetylcholinesterase (AChE) from Oreochromis mossambicus (Toman) as a sensitive test for the presence of insecticides. The partial purification and characterization of a soluble AChE from Oreochromis mossambicus brain tissues using affinity chromatography gel (procainamide–Sephacryl S-1000) showed that the partially purified AChE was most active on acetylthiocholine (ATC) but had low activities on propionylthiocholine (PTC) and butyrylthiocholine (BTC), indicating that the partially purified fraction was predominantly AChE. Soluble AChE was partially purified 9.27-fold with a 91.12% yield. The partially purified AChE displayed the highest activity on ATC at pH 7 and at 30oC using 0.1 M Tris buffer. The enzyme exhibited Michaelis-Menten kinetic constants, Km, for ATC, BTC and PTC at 36, 77 and 250 µM, respectively, and the maximum velocities, Vmax, were 18.75, 0.12 and 0.05 µmol/min/mg protein, respectively. Moreover, the AChE from Oreochromis mossambicus presented comparable sensitivity to carbamates and organophosphates insecticides than that from Electrophorus electricus and many other fish AChE by comparing half maximal inhibitory concentration values. Therefore, the enzyme is a valuable source for insecticides detection in Malaysian waters at lower cost. 
Published
2016-12-31
How to Cite
MUHAMMAD, Umar Abubakar et al. Assessment of acetylcholinesterase (AChE) from Oreochromis mossambicus (Cuvier, 1831) as a source of enzyme for insecticides detection. Bioremediation Science and Technology Research, [S.l.], v. 4, n. 2, p. 11-17, dec. 2016. ISSN 2289-5892. Available at: <http://journal.hibiscuspublisher.com/index.php/BSTR/article/view/370>. Date accessed: 19 july 2018.
Section
Articles