Isolation and culture medium optimisation using one-factor-at-time and Response Surface Methodology on the biodegradation of the azo-dye amaranth

  • Noris Kartika Sari Islahuddin Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Izuan Effendi Halmi Department of Land Management, Faculty of Agriculture, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Motharasan Manogaran Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
  • Mohd Yunus Shukor Dept of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.

Abstract

Isolate JR1 was isolated from the polluted textile industry activities site in the Juru Penang area. This bacterium was characterized as a gram-positive Bacillus bacterium and also gave a positive biochemical test for catalase test and oxidase test. The isolate JR1 gave a maximum decolourization of Amaranth dye under static conditions with the rate of decolorization of 98.82%. Seven variables which are pH, temperature (°C), ammonium acetate (g/L), glucose (g/L), sodium chloride (g/L), yeast (g/L) and dye concentration (ppm) was run by using Plackett-Burman design for the effective parameter of the decolourization of Amaranth. From the seven variables, three effective variables which were ammonium acetate, glucose, and dye concentration were further optimized by using a central composite design. The optimum value of ammonium acetate concentration at 0.74 g/L, glucose concentration at 3.0 g/L and a dye concentration at 58.1 ppm gave the highest percentage of decolourization. Thus, this isolate could provide an alternate solution in removing toxic dyes from environments.
Published
2017-12-31
How to Cite
ISLAHUDDIN, Noris Kartika Sari et al. Isolation and culture medium optimisation using one-factor-at-time and Response Surface Methodology on the biodegradation of the azo-dye amaranth. Bioremediation Science and Technology Research, [S.l.], v. 5, n. 2, p. 25-31, dec. 2017. ISSN 2289-5892. Available at: <http://journal.hibiscuspublisher.com/index.php/BSTR/article/view/360>. Date accessed: 21 may 2018.
Section
Articles