A one dimensional PAGE studies on <i>Puntius javanicus</i> liver proteome affected by copper toxicity

Authors

  • Mohd Khalizan Sabullah Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Fisal Ahmad School of Food Science & Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
  • Azlan Jualang Gansau Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Mohd Rosni Sulaiman Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v3i2.297

Keywords:

Puntius javanicus, 1-D PAGE, native PAGE, SDS-PAGE, copper

Abstract

Observations on the effects of copper on the liver proteome of Puntius javanicus  based on the one dimensional PAGE was carried out. The liver was dissected from each fish, which was separately treated with different concentrations of copper sulfate ranging from 0.1 to 5.0 mg/L. The livers were extracted and one dimensional PAGE was performed under nonreducing (native) and reducing (SDS)-PAGE. Several bands were resolved in the native PAGE with probable candidates for the effect of copper observed showing an increased in the expression and downregulation strongly associated with increasing copper concentrations. This study showed that high concentrations of copper significantly alters P. Javanicus liver at the proteome level, and preliminary screening based on one dimensional PAGE is considered rapid and simple to assess the toxicity effect of copper before more advanced and extensive assesment with a second dimensional PAGE is carried out.

References

Sabullah MK, Sulaiman MR, Abd Shukor MY, Shamaan NA, Khalid A, Ahmad SA. In vitro and in vivo effects of Puntius javanicus cholinesterase by copper. Fresenius Environ Bull. 2015;24(12 B):4615–4621.

Shukor MY, Bakar NA, Othman AR, Yunus I, Shamaan NA, Syed MA. Development of an inhibitive enzyme assay for copper. J Environ Biol. 2009;30(1):39–44.

Venkateswara Rao J, Begum G, Pallela R, Usman PK, Nageswara Rao R. Changes in behavior and brain acetylcholinesterase activity in mosquito fish, Gambusia affinis in response to the sub-lethal exposure to chlorpyrifos. Int J Environ Res Public Health. 2005;2(3–4):478–83.

Tilton FA, Bammler TK, Gallagher EP. Swimming impairment and acetylcholinesterase inhibition in zebrafish exposed to copper or chlorpyrifos separately, or as mixtures. Comp Biochem Physiol Toxicol Pharmacol CBP. 2011 Jan;153(1):9–16.

Sabullah MK, Shukor MY, Sulaiman MR, Shamaan NA, Syed MA, Khalid A, et al. The effect of copper on the ultrastructure of Puntius javanicus hepatocyte. Aust J Basic Appl Sci. 2014;8(15):245–51.

Sabullah MK, Sulaiman MR, Shukor MYA, Syed MA, Shamaan NA, Khalid A, et al. The assessment of cholinesterase from the liver of Puntius javanicus as detection of metal ions. Sci World J. 2014;2014.

Huang Q, Huang H-Q. Alterations of protein profile in zebrafish liver cells exposed to methyl parathion: a membrane proteomics approach. Chemosphere. 2012 Mar;87(1):68–76.

Rodrigues PM, Silva TS, Dias J, Jessen F. PROTEOMICS in aquaculture: applications and trends. J Proteomics. 2012;75(14):4325–45.

Forné I, Abián J, Cerdà J. Fish proteome analysis: model organisms and non-sequenced species. Proteomics. 2010 Feb;10(4):858–72.

Sabullah MK, Ahmad SA, Hussin J, Gansau AJ, Sulaiman MR. Acute effect of copper on Puntius javanicus survival and a current opinion for future biomarker development. J Environ Bioremediation Toxicol. 2014;2(1):28–32.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1–2):248–54.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227(5259):680–5.

Yan JX, Wait R, Berkelman T, Harry RA, Westbrook JA, Wheeler CH, et al. A modified silver staining protocol for visualization of proteins compatible with matrix-assisted laser desorption/ionization and electrospray ionization-mass spectrometry. Electrophoresis. 2000 Nov;21(17):3666–72.

Datta P, Chakrabarty S, Chakrabarty A, Chakrabarti A. Spectrin interactions with globin chains in the presence of phosphate metabolites and hydrogen peroxide: implications for thalassaemia. J Biosci. 2007 Sep;32(6):1147–51.

Wang M, Wang Y, Wang J, Lin L, Hong H, Wang D. Proteome profiles in medaka (Oryzias melastigma) liver and brain experimentally exposed to acute inorganic mercury. Aquat Toxicol Amst Neth. 2011;103(3–4):129–39.

Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007;35(4):495–516.

Chen D, Chan KM. Identification of hepatic copper-binding proteins from tilapia by column chromatography with proteomic approaches. Met Integr Biometal Sci. 2012 Aug;4(8):820–34.

Hartwig A. Zinc finger proteins as potential targets for toxic metal ions: differential effects on structure and function. Antioxid Redox Signal. 2001 Aug;3(4):625–34.

Aiba I, Hossain A, Kuo MT. Elevated GSH level increases cadmium resistance through down-regulation of Sp1-dependent expression of the cadmium transporter ZIP8. Mol Pharmacol. 2008 Sep;74(3):823–33.

Downloads

Published

2015-12-15

How to Cite

Sabullah, M. K., Ahmad, F., Gansau, A. J., & Sulaiman, M. R. (2015). A one dimensional PAGE studies on <i>Puntius javanicus</i> liver proteome affected by copper toxicity. Bioremediation Science and Technology Research (e-ISSN 2289-5892), 3(2), 26–29. https://doi.org/10.54987/bstr.v3i2.297

Issue

Vol. 3 No. 2 (2015)

Section

Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).