A one dimensional PAGE studies on <i>Puntius javanicus</i> liver proteome affected by copper toxicity

Authors

  • Mohd Khalizan Sabullah Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Fisal Ahmad School of Food Science & Nutrition, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
  • Azlan Jualang Gansau Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Mohd Rosni Sulaiman Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Sabah, Malaysia.

DOI:

https://doi.org/10.54987/bstr.v3i2.297

Keywords:

Puntius javanicus, 1-D PAGE, native PAGE, SDS-PAGE, copper

Abstract

Observations on the effects of copper on the liver proteome of Puntius javanicus  based on the one dimensional PAGE was carried out. The liver was dissected from each fish, which was separately treated with different concentrations of copper sulfate ranging from 0.1 to 5.0 mg/L. The livers were extracted and one dimensional PAGE was performed under nonreducing (native) and reducing (SDS)-PAGE. Several bands were resolved in the native PAGE with probable candidates for the effect of copper observed showing an increased in the expression and downregulation strongly associated with increasing copper concentrations. This study showed that high concentrations of copper significantly alters P. Javanicus liver at the proteome level, and preliminary screening based on one dimensional PAGE is considered rapid and simple to assess the toxicity effect of copper before more advanced and extensive assesment with a second dimensional PAGE is carried out.

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Published

2015-12-15

How to Cite

Sabullah, M. K., Ahmad, F., Gansau, A. J., & Sulaiman, M. R. (2015). A one dimensional PAGE studies on <i>Puntius javanicus</i> liver proteome affected by copper toxicity. Bioremediation Science and Technology Research, 3(2), 26–29. https://doi.org/10.54987/bstr.v3i2.297

Issue

Vol. 3 No. 2 (2015)

Section

Articles

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